Hantavirus IgG and IgM Antibodies (Sendout)

General Information

Lab Name

Lab Code

228

External Test Id

HANGM

Description

Useful For: Aiding in the diagnosis of Hantavirus infection.

Highlights: This test detects IgM and IgG class antibodies in serum against two Hantavirus strains, Sin Nombre virus and Andes virus. However, it does not differentiate between the two strains.

Ordering Requirements: This test requires approval from the Laboratory Medicine Resident (LMR) on-call. Contact the LMR via the paging officer at 206-598-6190.

References

Mayo Clinical & Interpretive Information: Hantavirus IgM and IgG, ELISA, Serum

Synonyms

Andes Virus, FHVGM, HANGM, Hantavirus Antibody, Hantavirus Pulmonary Syndrome, Hemorrhagic Fever with Renal Syndrome, Sin Nombre Virus

Interpretation

Method

Enzyme-Linked Immunosorbent Assay (ELISA)

Reference Range

Ref. Range Notes

Reference Values:

IgM:	Negative
IgG:	Negative

Interpretation:

IgM result	IgG result	Interpretation
Negative	Negative	No antibodies to Hantavirus (Sin Nombre or Andes strains) detected. False-negative results may occur in patients tested too soon following infection or in immunocompromised patients.
Negative	Borderline	Possible detection of IgG against Hantavirus (Sin Nombre or Andes strains, unable to differentiate). Consider repeat testing on a new specimen to confirm.
Negative	Positive	IgG against Hantavirus (Sin Nombre or Andes strains, unable to differentiate) detected. Results suggest prior exposure or infection with Hantavirus.
Borderline	Negative	Possible detection of IgM against Hantavirus (Sin Nombre or Andes strains, unable to differentiate). Consider repeat testing on a new specimen to confirm.
Borderline	Borderline	Possible detection of IgM and IgG against Hantavirus (Sin Nombre or Andes strains, unable to differentiate). Consider repeat testing on a new specimen to confirm.
Borderline	Positive	IgG against Hantavirus (Sin Nombre or Andes strains, unable to differentiate) detected. Possible detection of IgM against Hantavirus (Sin Nombre or Andes strains, unable to differentiate). Consider repeat testing on a new specimen to confirm IgM results.
Positive	Negative	IgM against Hantavirus (Sin Nombre or Andes strains, unable to differentiate) detected. Results suggest recent infection with Hantavirus.
Positive	Borderline	IgM against Hantavirus (Sin Nombre or Andes strains, unable to differentiate) detected. Possible detection of IgG against Hantavirus (Sin Nombre or Andes strains, unable to differentiate). Consider repeat testing on a new specimen to confirm IgG results.
Positive	Positive	IgM and IgG against Hantavirus (Sin Nombre or Andes strains, unable to differentiate) detected. Results suggest recent infection with Hantavirus.

Interferences and Limitations

Cautions:

False-negative results may occur in patients that are tested either too soon following infection or are significantly immunocompromised.

This assay detects IgM and IgG antibodies against antigens from the Sin Nombre and Andes Hantavirus strains; however, it will not differentiate between the two strains. It is unknown if this assay will detect antibodies to other Hantavirus strains.

Results must be interpreted alongside epidemiological exposure history and clinical presentation and diagnosis should not be made solely on serologic results from this assay.

False-positive results may occur in patients with recent cytomegalovirus infection.

Results from this assay are qualitative only.

Guidelines

Washington DOH Notifiable Conditions

Ordering & Collection

Specimen Type

Blood

Collection

3 mL blood in GOLD SST (preferred) or RED TOP tube

Approval Required

Laboratory Medicine Resident (LMR) approval required.

Handling Instructions

Outside Laboratories: Centrifuge sample and aliquot serum into a separate plastic vial. Refrigerate serum while awaiting shipment. Transport with a cold pack.

Stability: Refrigerated (preferred): 21 days; Frozen: 30 days; Ambient: 7 days.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, heat-inactivated specimen.

Quantity

requested: 0.6 mL serum
minimum: 0.5 mL serum

Processing

Centrifuge and aliquot serum into a separate vial. Refrigerate serum.

Login: SEND1-;REFRIG SERUM

RSNDT1: MAYOSD
RSTYP1: SRM
RTSRQ1: ;Hantavirus IgG and IgM Antibodies (Mayo Test HANGM)

Sendouts:

Order Mayo Test: HANGM