Laboratory Test Guide

Borrelia Species by PCR (Sendout)

General Information

Lab Name

Lab Code

260

External Test Id

0055570

Description

Ordering Recommendation: Not a first-line test for Lyme disease. May be useful if strong suspicion of Lyme disease persists in spite of persistent negative serologic testing. Blood and CSF specimens have poor clinical sensitivity for detection of Borrelia burgdorferi by PCR.

For Lyme Disease Serology testing, refer to Lyme Disease Serology w/ Conf (Sendout) [RLYMES].

Synonyms

0055570, B. burgdorferi DNA detection, Borrelia burgdorferi, Lyme Disease, Lyme Disease PCR, Tick Borne Disease

Interpretation

Method

Qualitative Polymerase Chain Reaction

Ref. Range Notes

Reference Range: Not Detected

Guidelines

Washington DOH Notifiable Conditions

Ordering & Collection

Specimen Type

Blood, CSF, Tissue, Synovial Fluid

Collection

Blood: collect 5 mL in LAVENDER TOP (EDTA) tube or GOLD SST

Unacceptable: Greent top (heparin) tubes

CSF or Synovial fluid: 1 mL fluid in a sterile container

Tissue: Transfer tissue to a sterile container

Handling Instructions

Outside Laboratories:

Blood: Centrifuge sample and transfer serum or plasma to a separate sterile aliquot. Freeze at -20°C while awaiting shipment.
CSF or Synovial Fluid: Transfer 1 mL CSF to a sterile aliquot. Freeze at -20°C while awaiting shipment.
Tissue: Transfer tissue to a sterile container and freeze at -20°C immediately.

Transport samples on dry ice. Indicate the specimen type on the UW requisition.

Stability:

Serum/Plasma/CSF/Synovial Fluid: Frozen (preferred): 1 year; Refrigerated: 72 hours; Ambient: 8 hours.
Tissue: Frozen (required): 1 year; Refrigerated: Unacceptable; Ambient: Unacceptable.

Unacceptable Conditions: Heparinized specimens, tissues in optimal cutting temperature compound.

Quantity

requested: 1 mL CSF/Synovial Fluid/Serum/Plasma
minimum: 0.5 mL CSF/Synovial Fluid/Serum/Plasma

Processing

Processing:

Blood: Centrifuge sample and transfer serum or plasma to a separate sterile aliquot. Freeze at -20°C.
CSF or Synovial Fluid: Transfer 1 mL CSF to a sterile aliquot. Freeze at -20°C.
Tissue: Transfer to a sterile container and freeze at -20°C immediately.

Sendouts:

Order ARUP Test: 0055570
Interfaced: Yes [Interface: 602; Department: ARPZ]

Stability:

Serum/Plasma/CSF/Synovial Fluid: Frozen (preferred): 1 year; Refrigerated: 72 hours; Ambient: 8 hours.
Tissue: Frozen (required): 1 year; Refrigerated: Unacceptable; Ambient: Unacceptable.

Unacceptable Conditions: Heparinized specimens, tissues in optimal cutting temperature compound.

Use the login information directly below for tissue samples only. For other sample types, refer to the information under Misc. Sendout.

Login (Tissue Samples Only):

Test Code	Modifier
RARUPZ	N/A

Result-at-entry (Tissue Samples Only):

Component	Enter	Type of Entry
RARUZN (Test Name)	;Borrelia Species by PCR (Sendout)	Free Text
RARUZS (Specimen Type)	TIS	Text Code
RARUZT (ARUP Test Code)	0055570	Numeric

Performance

LIS Dept Code

Performing Location(s)

Sendout	ARUP Laboratories, Inc. 800-522-2787 500 Chipeta Way Salt Lake City, UT 84108