Laboratory Test Guide

Tissue Plasminogen Activator Antigen (Sendout)

General Information

Lab Name

Lab Code

607

External Test Id

0099187

Description

Ordering Recommendation:

Determine quantity of tissue plasminogen ( tPA) in plasma.
May be helpful in detecting disorders of the fibrinolytic system.
Not a first-line test for diagnosing inherited thrombotic or bleeding disorders.

Synonyms

0099187, TPA Antigen, TPAAG

Interpretation

Method

Enzyme-Linked Immunosorbent Assay

Ref. Range Notes

Reference Range: ≤12.4 ng/mL

Ordering & Collection

Specimen Type

Plasma

Collection

5 mL blood in BLUE TOP (3.2% Sodium Citrate) Tube

Note: If no other samples are to be collected, draw a discard (non-additive tube) tube prior to drawing coagulation specimens.

Expedite transport to the laboratory. The lab must receive and process specimen within 2 hours of collection.

Handling Instructions

Outside Laboratories:

Centrifuge sample for 10 minutes and remove plasma.
Centrifuge plasma for another 10 minutes.
Transfer 1 mL plasma (0.5 mL minimum) to a separate plastic vial and freeze at -20°C to -80°C.
Send sample frozen on dry ice.

Stability: Frozen: 2 months; Refrigerated: Unacceptable; Ambient: 4 hours.

Unacceptable Conditions: Serum, EDTA plasma, clotted or hemolyzed specimens.

Critical Frozen. Separate specimens must be submitted when multiple tests are ordered.

Quantity

requested: 1 mL sodium citrate plasma
minimum: 0.5 mL sodium citrate plasma

Processing

UW-MT & HMC Instructions: Forward specimen to Coag bench for processing.

Coagulation: Within 2 hours of collection, double-centrifuge sample and transfer 1 mL (0.5 mL min.) plasma to a separate plastic vial. Freeze plasma at -70°C (Sendouts frozen rack).

Sendouts:

Order ARUP Test: 0099187
Interfaced: Yes [Interface: 602; Department: ARPZ]