Laboratory Test Guide

Plasminogen Activator Inhibitor Antigen 1 (PAI-1)

General Information

Lab Name

Lab Code

608

External Test Id

36555

Description

Clinical Significance: Increased activity is associated with increased risk of arterial thrombosis, such as with unexplained premature myocardial infarction. As an acute phase reactant, the activity is increased after an acute event. Studies suggest PAI-1 may be a prognostic marker in early stage breast cancer.

Synonyms

36555, 59766P, PAI-1, PAI1, PAIAG

Interpretation

Method

Enzyme Immunoassay (EIA)

Ref. Range Notes

Reference Range: 4-43 ng/mL

Ordering & Collection

Specimen Type

Plasma

Collection

5 mL blood in BLUE TOP (3.2 % Sodium Citrate) Tube

Note: If no other samples are to be collected, draw a discard (non-additive tube) tube prior to drawing coagulation specimens.

Expedite transport to the laboratory. The lab must receive and process specimen within 2 hours of blood collection.

Handling Instructions

Outside Laboratories:

Centrifuge sample for 15 minutes.
Remove plasma and centrifuge plasma for another 15 minutes.
Transfer plasma into a separate plastic vial within 2 hours of collection using a plastic Pasteur pipette. Do not use glass tubes or glass Pasteur pipettes, as glass can activate the clotting cascade. Freeze plasma (minimum 0.5 mL) at -20°C to -80°C.
Send Frozen on dry ice.

Stability: Frozen (-70°C): 1 year; Frozen (-20°C): 30 days; Refrigerated: 2 hours; Ambient: Unacceptable.

Reject Criteria: Received refrigerated or ambient.

Quantity

requested: 1 mL sodium citrate plasma
minimum: 0.5 mL sodium citrate plasma

Processing

UW-MT & HMC Instructions: Forward specimen to Coag bench for processing.

Coagulation: Within 2 hours of collection, double-centrifuge sample and transfer 1 mL (0.5 mL min.) plasma to a separate plastic vial. Freeze plasma at -70°C (Sendouts frozen rack).

Login: SEND1-;FREEZE

RSNDT1: QNICH
RSTYP1: PLS
RTSRQ1: ;Plasminogen Activator Inhibitor Antigen (Quest Test 36555)

Sendouts, order Quest Test: 36555.