Adenoviruses by PCR Qualitative, Tissue or Cells

General Information

Lab Name

ADV Qualitative PCR, Tissue/Cells

Lab Code

ADVQLT

Epic Name

ADV Qualitative PCR, Tissue/Cells

Description

The detection and quantification of Adenoviruses by real-time PCR amplification. The assay is a multiplex assay divided into 2 reactions. PCR reaction “1” detects amplicons of the hexon region for subgroups A & F. PCR reaction “2” detects amplicons of the hexon region of subgroups B,D,&E and the penton region for subgroup C.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood or CSF Samples, see Adenoviruses, PCR Quantitative [ADVQN].

For Urine, see Adenovirus by PCR Quantitative, Urine [UADVQN].

Components

Code	Name
ADVQLS	ADV Qual Specimen Descrip
ADVQLA	ADV A, F Qual Result
ADVQLB	ADV B, C, D, E Qual Result
ADVQLI	ADV Qual Interp

Interpretation

Method

Real time Polymerase Chain Reaction.

Reference Range

See individual components

Ref. Range Notes

Not applicable.

Ordering & Collection

Specimen Type

Tissue Biopsy, Paraffin Tissue, Bone Marrow, Swab or Cellular fluid (e.g. BAL or Washing)

Collection

Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).

Paraffin Tissue: DO NOT SEND SLIDES. Slice 3-4 scrolls that are 5-50 micron thick in sterile tube.

Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube. No Heparin aspirate in syringe.

Swab: In sterile tube/universal transport media (UTM).

Cellular fluid: 1 mL in sterile tube

Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.

Minimum sample requirement: 0.5 mL

Handling Instructions

Bone Marrow: 5 mL bone marrow in EDTA (LAVENDER TOP) tube to Laboratory at room temperature within 24 hours of collection.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Quantity

requested: 1 mL
minimum: 0.5 mL

Processing

Tissue Biopsy: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Paraffin Tissue: Ship at room temperature.

Bone Marrow: Ship at room temperature within 24 hours of collection.

Swabs: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory:

Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder.

Tissue/Cellular fluid/Swab: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code

Central Lab Virology (RVIR)

Performing Location(s)

CL	Infectious Disease Division (IDD) - Virology 1601 Lind Ave SW Renton, WA 98057	Phone: 206-685-6656 option 8 Hours of Operation: Mon–Fri: 7:00 AM–7:00 PM Sat: 9:00 AM–4:00 PM

Laboratory Test Guide