Anti Endomysial, IgA (by IFA)
General Information
Lab Name
Anti Endomysial, IgA
Lab Code
AEMYA
Epic Name
Anti Endomysial, IgA
Description
The presence of IgA endomysial autoantibodies by indirect immunofluorescence is suggestive of active gluten sensitive enteropathies such as celiac disease. Results are primarily useful for diagnosis; they may also be used for monitoring response to treatment with a gluten-free diet, but results are semi-quantitative.
References
Bürgin-Wolff, et al., Antigliadin and antiendomysium antibody determination for coeliac disease. Arch Dis Child. (1991) 66:941.
Synonyms
Celiac, Celiac Ab, Endomysium, Sprue
Interpretation
Method
Indirect immunofluorescence (IFA) using INOVA monkey esophagus tissue to detect IgA autoantibodies.
Reference Range
| Female | Male | ||
|---|---|---|---|
| Age | Range | Age | Range |
| 0- | Negative [NRN] | 0- | Negative [NRN] |
Effective date: 04/01/2010
Ref. Range Notes
Established by testing healthy blood donors during method validation.
Interferences and Limitations
Anti-smooth muscle antibodies may obscure endomysial staining. The test cannot be used for patients with IgA deficiency.
Grossly hemolyzed or lipemic serum may produce increased nonspecific background staining of substrate.
Guidelines
Ordering & Collection
Specimen Type
Collection
3 mL blood in RED TOP or SST tube
Handling Instructions
Instructions for Outside Clients: Freeze serum at -20°C
Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C: Long Term
Quantity
requested: 1 mL serum
minimum: 0.3 mL serum
Processing
Freeze serum at -20°C
Note: If Anti Endomysial is requested without specifying method (i.e. IFA or EIA), then order: AEMYA
Note2: AEMYA may be reflexively added on to CELRP orders if indicated.
Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C: Long Term
Performance
LIS Dept Code
Immunology (IMM)
Performing Location(s)
| UW-MT |
Immunology
206-520-4600 Clinical Lab, Room NW220, |
|---|
Frequency
Wednesday, Friday. Negative results same day. Positive results on next run.
Available STAT?
No