AFB Culture, Blood
General Information
Lab Name
AFB Culture, Blood
Lab Code
AFBBC
Epic Name
AFB Culture, Blood
Description
See AFB Culture, Bone Marrow [AFBBNM] for AFB culture on bone marrow.
Synonyms
Acid Fast, AFB, MAC, MTB, Mycobacteria, TB, Tuberculosis
Components
| Code | Name |
|---|---|
| SDES | Specimen Description |
| SREQ | Special Requests |
| CULT | Culture |
| RPT | Report Status |
Interpretation
Method
BD BACTEC™ Myco/F Lytic culture vials
BD BACTEC™ FX40 System for Mycobacteria Blood Cultures
Reference Range
See individual componentsOrdering & Collection
Specimen Type
Collection
Disinfect draw site and TUBE TOP. Allow disinfectant to dry. Collect blood in a yellow-top BD Vacutainer Tube containing SPS. Do NOT overfill or underfill. Inoculate the maximum volume for optimal sensitivity of culture.
Unacceptable:
- Any anticoagulant other than SPS
- Specimens older than 15 hrs will be rejected
HMC: Contact HMC Microbiology at 206-744-5858 for SPS tube. Refer to "Nursing Services Policy and Procedures for Nursing Care: Obtaining Blood Culture" procedure.
UW-MT: SPS tubes are available from Material Management. Refer to "Nursing Services Policy and Procedures for Nursing Care: Obtaining Blood Culture" procedure. Specimen will be sent to HMC for testing.
UW-NW: Contact UW-NW specimen processing at 206-668-2026 for SPS tube. Specimen will be sent to HMC for testing.
Handling Instructions
Transport at room temperature to Microbiology laboratory within 30 minutes after collection. Specimens older than 15 hours will be rejected. Specimen must be received in HMC Microbiology BEFORE 10:30 pm for same day processing.
Quantity
requested: 8.3 mL blood
minimum: 1 mL Blood
Processing
Specimens received from outside locations and clinics must be sent to the HMC Microbiology lab immediately for processing. At login, enter the phlebotomy site.
Performance
LIS Dept Code
Microbiology (MC)
Performing Location(s)
| HMC |
Microbiology
206-744-5858 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Other Locations/Notes
HMC Microbiology: Inoculate blood into a BACTEC Myco/F Lytic culture vial. See lab procedure “BD BACTED FX40 System for Mycobacteria Blood Cultures” for further directions.
Frequency
Daily: 7:00 - 22:30 [Midnight Shift: leave SPS tube at room temperature and leave a message to alert Dayshift Microbiology.]
Available STAT?
No