AFB Culture, Bone Marrow
General Information
Lab Name
AFB Culture, Bone Marrow
Lab Code
AFBBNM
Epic Name
AFB Culture, Bone Marrow
Description
See Lab Mnemonic AFB Culture, Blood [AFBBC] for AFB culture on adult blood.
Synonyms
Acid Fast, AFB, Bone Marrow, MAC, MTB, Mycobacteria, TB, Tuberculosis
Components
| Code | Name |
|---|---|
| SDES | Specimen Description |
| SREQ | Special Requests |
| CULT | Culture |
| RPT | Report Status |
Interpretation
Method
BD BACTEC™ Myco/F Lytic Culture Vials
BD BACTEC™ FX40 System for Mycobacteria Blood Cultures
Reference Range
See individual componentsOrdering & Collection
Specimen Type
Collection
Disinfect draw site AND TUBE TOP. Allow disinfectant to dry. Do NOT overfill or underfill. Inoculate the maximum volume for optimal sensitivity of culture.
Collect bone marrow in BD Vacutainer SPS tube
If an AFB stain from bone marrow is also requested, prepare two smears. AFB Direct Stain [AFBST]
HMC: Contact HMC Microbiology at 206-744-5858 for SPS tube.
UW-MT: Contact UW-MT Microbiology at 206-598-6147 for SPS tube. Specimen will be sent to HMC for testing.
UW-NW: Contact UW-NW specimen processing at 206-668-2026 for SPS tube. Specimen will be sent to HMC for testing.
Unacceptable: Any anticoagulant other than SPS.
Handling Instructions
Transport at room temperature to HMC Microbiology laboratory ASAP.
Specimens older than 15 hours will be rejected.
Specimen must be received in HMC Microbiology BEFORE 10:30 pm for same day processing
If an AFB stain is requested, transport 2 smears in a slide container taped closed and labeled BIOHAZARD.
Quantity
requested: 1.5 mL Bone Marrow
minimum: 0.75 mL Bone Marrow
Processing
At login, enter the phlebotomy site. If a bone marrow AFB stain is requested AND stains were received, also log in AFBST.
Performance
LIS Dept Code
Microbiology (MC)
Performing Location(s)
| HMC |
Microbiology
206-744-5858 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Frequency
Daily: 7:00 - 22:30 [Midnight Shift: leave SPS tube at room temperature and leave a message to alert Dayshift Microbiology.]
Available STAT?
No