Anti Neutrophil Cytoplasmic Antibody
General Information
Lab Name
Antineutrophil Cytoplasmic Antibody by IF
Lab Code
ANCAIF
Epic Name
Antineutrophil Cytoplasmic Antibody by IF
Description
For neutrophil surface antibodies, see information under Lab Mnemonic: 102
Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.
Synonyms
ANCA by IF, ANCA by Immunofluorescence
Interpretation
Method
Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.
Reference Range
| Female | Male | ||
|---|---|---|---|
| Age | Range | Age | Range |
| 0- | Negative [NRN] | 0- | Negative [NRN] |
Effective date: 04/01/2010
Ref. Range Notes
Established by testing healthy blood donors during method validation.
Interferences and Limitations
Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.
Ordering & Collection
Specimen Type
Collection
3 mL blood in RED TOP or SST tube
Quantity
requested: 1 mL serum
minimum: 0.5 mL serum
Processing
Refrigerate serum. Stable 14 days refrigerated. Stable long term frozen.
SPS, Do NOT use this mnemonic UNLESS the request specifies 'ANCA by IF or ANCA by Immunofluorescence'.
See: ANCAP for regular logins of ANCA's.
Performance
LIS Dept Code
Immunology (IMM)
Performing Location(s)
| UW-MT |
Immunology
206-520-4600 Clinical Lab, Room NW220, |
|---|
Frequency
Monday-Friday
Available STAT?
See ANCAP or PRFAIL for STAT options