BK Virus by PCR Quantitative
General Information
Lab Name
BK Virus PCR Quant
Lab Code
BKVQN
Epic Name
BK Virus PCR Quant
Description
Blood (EDTA Plasma)
The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the FDA approved Roche cobas® BKV Quantitative assay for the detection of BK polyomavirus (BKV) using real-time PCR amplification.
The lower limit of detection, LoD (the minimum virus level that gives a positive result in 95% of replicates), and the lower limit of quantitation, LLoQ, are both 21.5 IU/mL.
A conversion factor of 1.1 Log10 IU/mL is equivalent to 1.0 Log10 copies/mL.
The Roche cobas® BKV assay uses primers specific for the BKV VP2 region and the BKV small t-antigen region.
For tissue biopsy, bone marrow, or cellular/viscous fluids (e.g., BAL or washings), refer to BK Virus by PCR Qualitative [BKVQL].
For Urine, refer to BK Virus by PCR Quantitative, Urine [UBKQN].
Synonyms
Polyomavirus Type BK-Quant
Components
| Code | Name |
|---|---|
| BKVQNS | BK Virus Quant Specimen Type |
| BKVQNR | BK Virus Quant Result |
| BKLOG | BK Virus log IU/mL |
| BKVQNI | BK Virus Quant Interpretation |
Interpretation
Method
Real-time Polymerase Chain Reaction
Reference Range
See individual componentsRef. Range Notes
Not Applicable
Guidelines
Ordering & Collection
Specimen Type
Collection
Blood: 3 mL EDTA plasma or PPT
Unacceptable samples: Plasma collected in sodium or lithium heparin.
Note: Separate specimens MUST be submitted when both Serology and PCR testing are ordered.
Handling Instructions
Freshly drawn whole blood specimens may be held at 2 to 25°C for up to 24 hours prior to plasma preparation through centrifugation.
Quantity
requested: 1.0 mL of EDTA plasma
minimum: 350µL of EDTA plasma
Processing
Blood: Centrifuge upon arrival, remove plasma from cells.
Plasma specimens may be stored and/or transported:
- 2 to 30°C for up to 24 hours
- 2 to 8°C for up to 6 days
- -15°C to -80°C for long-term storage up to 6 months.
Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is unavailable, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 6 days of collection. Do not freeze/thaw.
Performance
LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
| CL |
Division of Infectious Disease Diagnostics (DIDD) - Virology
1601 Lind Ave SW |
Phone: 206-685-6656 option 8 |
|---|
Frequency
Tuesday and Friday. Results same day.
Available STAT?
No