CMV Quant by PCR, Blood Spots

Dried Blood Spots

The detection and quantitation of CMV by real-time PCR amplification. PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Quantitation of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is equivalent to 300 IU/mL (2.48 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF or Clear non-cellular fluid (e.g. BAL or Washing), see CMV Quantitative by PCR [CMVQN].

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (e.g. BAL or Washing), see CMV Qualitative by PCR, Tissue/Cells [CMVQLT].

For Eye Fluid, see CMV Quant PCR for Eye Fluids [CMVEYE] or Eye Fluid Viral PCR Quant Panel [EYEVQP].