CYP24A1 Deficiency Screen
General Information
Lab Name
CYP24A1 Deficiency Screen
Lab Code
CYP24A
Epic Name
CYP24A1 Deficiency Screen
Description
The concentrations of five vitamin D metabolites are determined by immunoaffinity extraction followed by liquid chromatography-tandem mass spectrometry. The reportable range is 5-200 pg/mL for both 1,25(OH)2D2 and 1,25(OH)2D3, 0.1 to 100.0 ng/mL for 24,25(OH)2D3, 1.0 to 200.0 ng/mL for both 25(OH)D2 and 25(OH)D3. with imprecision between 8.7 and 13.0 %CV, 5.1 and 6.0 %CV, and 4.5 and 8.0 %CV, respectively.
Synonyms
Dihydroxyvitamin D
Components
| Code | Name |
|---|---|
| IVITDM | CYP24A1 Screen IA_MS Method |
| 125VD2 | 1,25 Dihydroxy Vitamin D2 |
| 125VD3 | 1,25 Dihydroxy Vitamin D3 |
| 125VDT | 1,25 Dihydroxy Vitamin D Total |
| 2425VD | Vitamin D3 (24,25_Dihydroxy) |
| IVITD2 | Vitamin D2 (25_Hydroxy) |
| IVITD3 | Vitamin D3 (25_Hydroxy) |
| IVITDT | Vitamin D (25_Hydroxy) Total |
| 24DRIN | Vitamin D Ratio Information |
| 24DRRE | Vitamin D Ratio Result |
Interpretation
Method
Serum immunoaffinity extraction - Isotope dilution liquid chromatography mass spectrometry
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
All Locations:
Preferred: 4 mL blood in GOLD SST tube
Also Acceptable: 4 mL blood in ORANGE RST or RED TOP tube
Pediatric: 2 Full GOLD MICROTAINER
Quantity
requested: 1 mL serum
minimum: 0.8 mL serum
Processing
Freeze serum at -20°C
Performance
LIS Dept Code
Chemistry, University (CHU)
Performing Location(s)
| UW-MT |
Chemistry, General
206-520-4600 Clinical Lab, Room NW220, |
|---|
Frequency
Tuesday, results next day
Available STAT?
No