Protein Electrophoresis Reflexive Panel
General Information
Lab Name
Protein Electrophoresis Panel
Lab Code
ELPP
Epic Name
Protein Electrophoresis Reflexive Panel
Description
Purpose: This test can be used to screen for and identify a monoclonal protein (M-spike).
The initial test performed is serum protein electrophoresis. Samples with a monoclonal band present on protein electrophoresis that has previously been identified in this laboratory will be resulted with the concentration of the known M-spike. Samples demonstrating previously unidentified protein bands of concern will be reflexively tested by immunofixation, which generates an additional result and charge. If a monoclonal band is identified by immunofixation, its concentration will be reported.
Serum free light chain testing (FLC) testing is available as a separate order, and is not included in this panel.
Synonyms
Gammopathy, Immunofixation, M-Protein, MGUS, Monoclonal, Monoclonal antibody, Multiple myeloma, Myeloma, Serum electrophoresis, Serum protein electrophoresis, SPEP, Waldenstrom
Components
| Code | Name |
|---|---|
| ETP | Total Protein (Beckman) |
| ETPRD | Total Protein (Roche) |
| EALB | Albumin |
| EA1 | Alpha 1 |
| EA2 | Alpha 2 |
| EB | Beta |
| EG | Gamma |
| INTRPE | Electrophoresis Interp: |
| MC1ID | Monoclonal 1 ID, Serum |
| MC1QN | Monoclonal 1 Quant, Serum |
| MC2ID | Monoclonal 2 ID, Serum |
| MC2QN | Monoclonal 2 Quant, Serum |
Interpretation
Method
Agarose Electrophoresis (AEP)
Reference Range
See individual componentsInterferences and Limitations
Hemolysis may falsely elevate alpha-2 and beta components.
Stability: 1 week refrigerated; stable long-term if frozen.
Guidelines
Ordering & Collection
Specimen Type
Collection
3 mL blood in RED TOP or GOLD SST tube.
Pediatric: 2 full GOLD or RED TOP MICROTAINERS.
Quantity
requested: 0.5 mL serum ELP; 0.5 mL for TP
Processing
Refrigerate serum.
Make two aliquots: 0.5 mL to IMM for ELP and 0.5 mL to CHEM for ETP or ETPRD.
OK to share ELP and IFIX on one tube if short sample. Forward shared pink slip to Immunology.
Outside Laboratory: If Total Protein is provided, please free text "TP provided" after test ordered and provide a copy of the slip to Panel Bench and Immunology.
Performance
LIS Dept Code
Immunology (IMM)
Performing Location(s)
| CL |
Immunology Lab
206-520-4600 Room# 573 |
Hours of Operation: Monday–Friday, 8:00 AM–5:00 PM PST |
|---|---|---|
| CL |
Renton Core Lab
206-520-4600 Clinical Lab, Room 170 |
Frequency
Monday-Friday
Available STAT?
No