Protein Electrophoresis Reflexive Panel
General Information
Lab Name
Protein Electrophoresis Panel
Lab Code
ELPP
Epic Name
Protein Electrophoresis Reflexive Panel
Description
Purpose: This test can be used to screen for and identify a monoclonal protein (M-spike).
The initial test performed is serum protein electrophoresis. Samples with a monoclonal band present on protein electrophoresis that has previously been identified in this laboratory will be resulted with the concentration of the known M-spike. Samples demonstrating previously unidentified protein bands of concern will be reflexively tested by immunofixation, which generates an additional result and charge. If a monoclonal band is identified by immunofixation, its concentration will be reported.
Serum free light chain testing (FLC) testing is available as a separate order, and is not included in this panel.
Synonyms
Gammopathy, Immunofixation, M-Protein, MGUS, Monoclonal, Monoclonal antibody, Multiple myeloma, Myeloma, Serum electrophoresis, Serum protein electrophoresis, SPEP, Waldenstrom
Components
| Code | Name |
|---|---|
| ETP | Protein (Total) |
| EALB | Albumin |
| EA1 | Alpha 1 |
| EA2 | Alpha 2 |
| EB | Beta |
| EG | Gamma |
| INTRPE | Electrophoresis Interp: |
Interpretation
Method
Agarose Electrophoresis (AEP)
Reference Range
See individual components
Interferences and Limitations
Hemolysis may falsely elevate alpha-2 and beta components.
Stability: 1 week refrigerated; stable long-term if frozen.
Guidelines
Ordering & Collection
Specimen Type
Collection
3 mL blood in RED TOP or GOLD SST tube.
Pediatric: 2 full GOLD or RED TOP MICROTAINERS.
Quantity
requested: 0.5 mL serum ELP; 0.5 mL for TP
Processing
Refrigerate serum.
Make two aliquots: 0.5 mL to IMM for ELP and 0.5 mL to CHEM for ETP.
OK to share ELP and IFIX on one tube if short sample. Forward shared pink slip to Immunology.
Outside Laboratory: If Total Protein is provided please free text "TP provided" after test ordered, and provide a copy of the slip to Panel Bench and Immunology.
Performance
LIS Dept Code
Immunology (IMM)
Performing Location(s)
| UW-MT |
Immunology
206-520-4600 Clinical Lab, Room NW220, |
|---|
Frequency
Monday-Friday
Available STAT?
No