Emicizumab Level
General Information
Lab Name
Emicizumab Level
Lab Code
EMILVL
Epic Name
Emicizumab Level
Description
This assay measures the concentration of emicizumab (Hemlibra) in plasma using a modified one-stage Factor VIII assay calibrated against emicizumab. The steady-state trough plasma concentration of emicizumab is reported to be approximately 25-80 ug/mL (See: Adamkewicz J, Chen D. Effects and Interferences of Emicizumab, a Humanised Bispecific Antibody Mimicking Activated Factor VIII Cofactor Function, on Coagulation assays. Thromb Haemost. 2019 July;119:1084-1093)
The presence of endogenous Factor VIII could cause a small apparent increase in emicizumab concentration.
Synonyms
Hemlibra level
Components
| Code | Name |
|---|---|
| EMIPAT | Emicizumab Level |
| EMIMET | Emicizumab Method |
Interpretation
Method
STA clot-based
Reference Range
See individual components
Ordering & Collection
Specimen Type
Collection
3 mL or 5 mL BLUE TOP (Citrate) tube
Outside Laboratories: Centrifuge BLUE top for 10 minutes, remove plasma and re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) at -20°C to -80°C. Send frozen on dry ice.
Forms & Requisitions
Outside clients should fill and submit Coagulation Patient Clinical History Form: Coagulation Patient Clinical History Form
Handling Instructions
The Laboratory MUST process specimen within 4 hours of collection.
Quantity
requested: Entire sample
minimum: 1.0mL plasma
Processing
UW-MT: Take specimen to UW-MT Coag Lab for processing. Coag tech will freeze plasma sample for transport to HMC Coag.
HMC: Take specimen to HMC Coag Lab for processing.
Performance
LIS Dept Code
Coagulation (COAG)
Performing Location(s)
| HMC |
Coagulation, Special
206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Frequency
Run on Wednesday. Reported by end of day.
Available STAT?
Yes, with Lab Medicine Resident's approval