Laboratory Test Guide

Fluid Bacterial Culture and Sensitivity (Special Anaerobic) with Gram Stain

General Information

Lab Name

Fluid C/S w/Gram

Lab Code

FLDANC

Epic Name

CULTURE:BACT-FLD,ANAER AND GRAM STAIN

Description

This test is intended for the culture of sterile body fluids (other than urine, blood or CSF).

For CSF, see Place Holder for CSF Bacterial Culture and Sensitivity with Gram Stain [CSFPHC].

For specimens collected from drains (other than CSF), see Fluid Bacterial Culture and Sensitivity with Gram Stain [FLDC].

If > 3 mL sterile fluid is received, fluid will be inoculated into blood culture bottles as well as onto solid media. See Fluid Bacterial C/S in Blood Culture Bottles with Gram Stain [FLDGBC].

Synonyms

Anaerobic Fluid Culture, Fluid Anaerobic Culture

Components

Code	Name
SDES	Specimen Description
SREQ	Special Requests
GS	Gram Smear
CULT	Culture
RPT	Report Status

Interpretation

Reference Range

See individual components

Guidelines

Antibiograms and Antimicrobial Susceptibility Testing Information

Ordering & Collection

Specimen Type

Fluid from sterile site

Collection

Optimal: Collect fluid in syringe (remove needle, expel air and cap syringe) or in sterile, leak-proof container.

Swabs are accepted but not optimal: Preferred method of transport is ESwab. Gel swab is acceptable.

Unacceptable: Specimens collected in Universal Transport Medium (UTM); dry swabs; syringes with needles; leaking specimens, specimens collected with expired swabs/transport media.

Handling Instructions

Specify exact source of specimen and "R/O Anaerobes" for optimal workup

Fluid: Transport at room temperature within 2 hours of collection.

Swabs: Transport at room temperature within 4 hours of collection.

Cold temperature reduces the viability of anaerobes.

Specimens will be rejected if received > 24 hours after collection. Viability of organisms is significantly reduced after 24 hours.

If cell count is also requested, see Cell Count, Body Fluid [FCCNT] & send EDTA (lavender top) tube within 1 hour.

Quantity

requested: As much fluid as possible
minimum: 1 mL fluid, ESwab

Processing

If > 1 mL sterile fluid is received, order FLDGBC.

If specimen is collected from a drain (i.e. JP, Penrose, etc.), order FLDC UNLESS from newly placed or IR collected drain.

Anaerobes must be cultured ASAP to maintain viability.

HMC: ADDCON fee is added for centrifugation of specimens >2 mL.

Performance

LIS Dept Code

Microbiology (MC)

Performing Location(s)

HMC	Microbiology 206-744-5858 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
UW-MT	Microbiology 206-520-4600 Clinical Lab, Room # NW177, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Other Locations/Notes

UW-MT Microbiology performs testing for UW-MT and UW-NW.

Frequency

Processed upon receipt

Available STAT?

Yes-Gram

Billing & Coding

CPT codes

87015, 87070, 87075, 87205

Billing Comments

CPT for ADDCON 87015

LOINC

17956-4

Interfaced Order Code

UOW4069

Interfaced Result Code

UOW4069