Hemoglobin A2 Quantification by HPLC
General Information
Lab Name
Hemoglobin A2 Quant
Lab Code
HBA2
Epic Name
Hemoglobin A2 Quant
Description
Hb A2 is eluted from the column resin by a programmed buffer gradient of increasing ionic strength. Hb A2 measurement is best interpreted in conjunction with other testing in the Thalassemia Reflexive Panel and red cell indices and iron supply measurements.
Synonyms
HbA2, Hb A2
Interpretation
Method
High Performance Liquid Chromatography (HPLC)
Reference Range
Units: %
| Female | Male | ||
|---|---|---|---|
| Age | Range | Age | Range |
| 0-6m | <3.5 | 0-6m | <3.5 |
| 7m- | 2.3-3.5 | 7m- | 2.3-3.5 |
Effective date: 11/02/2012
Interferences and Limitations
Variant hemoglobins such as Hb S, Hb C, Hb D interfere with measurement of Hb A2, so Hb A2 is not reported in the presence of these variants. Hb E co-elutes with Hb A2 and thus the level reported is the sum of percentages of Hb E and Hb A2.
Guidelines
Ordering & Collection
Specimen Type
Collection
3 mL LAVENDER HEMOGARD tube
Pediatric draw: 3 LAVENDER TOP MICROTAINER
Quantity
requested: 3 mL whole blood
minimum: 1.5 mL whole blood
Processing
Refrigerate whole blood Stability: EDTA: Refrigerated, 7 days. Ambient, 2 days.
Unacceptable: Other anticoagulants, Frozen samples.
Performance
LIS Dept Code
Red Cell Disorders (RCD)
Performing Location(s)
| HMC |
Red Cell Disorders
206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Frequency
Monday - Friday
Available STAT?
No