Hemoglobin Electrophoresis (Isoelectric Focusing)
General Information
Lab Name
Hb Elect._Isoelectric Focusing
Lab Code
HBELEC
Epic Name
Hemoglobin Electrophoresis (Isoelectric Focusing)
Description
Hemoglobin electrophoresis alone is not adequate for diagnosing thalassemia. Electrophoresis is limited to identification without fractionation, quantification or interpretation. This test is useful for screening for sickle cell trait for athletes other than those on UW teams. For most red cell disorders, we suggest instead ordering "Hb Disorder Thalassemia Reflex Pnl w/CBC" [HBTHLI]. If a CBC was performed within 30 days, order "Hb Disorder Thalassemia Reflexive Panel" [HBTHAL]. Both panels include hb electrophoresis, reflexive testing for thalassemia and an interpretation.
Synonyms
HB ELP, Hemoglobinopathy, hemoglobinopathy, red cell, Sickle Cell Trait, Sickle Cell-Routine, Sickle Dex-Routine
Components
| Code | Name |
|---|---|
| HER | Hb Electrophoresis Result |
| HBDET | Hemoglobins Detected |
| HBCOM | Hb Elect. Comment |
Interpretation
Method
Isoelectric Focusing Electrophoresis (IEF)
Reference Range
See individual components
Ref. Range Notes
Within Normal Limits
Guidelines
Ordering & Collection
Specimen Type
Collection
3 mL LAVENDER HEMOGARD tube
Pediatric draw: 3 LAVENDER TOP MICROTAINER
Handling Instructions
For STAT requests from ED location, see [SICPRE] Sickle Screen, Stat, ED only.
Quantity
requested: 3 mL whole blood
minimum: 1.5 mL whole blood
Processing
Refrigerate entire sample. If sickle screen is ordered, but not from ED location, login HBELEC.
If request is for Quantitative HbS or Hb Variant Quant., for pre- or post-transfusion, login HBQVAR.
If request is for Quantitative HBF for hydroxyurea therapy, login HBF.
Stability: EDTA: Refrigerated, 7 days. Ambient, 2 days
Unacceptable: Other anticoagulants, Frozen specimen
Performance
LIS Dept Code
Red Cell Disorders (RCD)
Performing Location(s)
| HMC |
Red Cell Disorders
206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Frequency
Monday - Friday
Available STAT?
No