Hemoglobin F - HPLC Quantification
General Information
Lab Name
Hemoglobin F Quant by HPLC
Lab Code
HBF
Epic Name
Hemoglobin F Quant by HPLC
Description
Hb F is eluted from the column resin by a programmed buffer gradient of increasing ionic strength.
Hb F level is useful for following hydroxyurea therapy for sickle cell patients.
Hb F measurement is best interpreted in conjunction with other testing in the Thalassemia Reflexive Panel, red cell indices, patient demographics and other clinical factors.
Synonyms
Fetal Hemoglobin, Hb F, Hydroxyurea Follow-up, red cell
Interpretation
Method
High Performance Liquid Chromatography (HPLC)
Reference Range
Units: %
| Female | Male | ||
|---|---|---|---|
| Age | Range | Age | Range |
| 0-4m | 5-90 | 0-4m | 5-90 |
| 5m | 5-20 | 5m | 5-20 |
| 6m-8m | <8 | 6m-8m | <8 |
| 9m | <5 | 9m | <5 |
| 10m-3y | <3 | 10m-3y | <3 |
| 4y-10y | <2 | 4y-10y | <2 |
| 11y- | <1.1 | 11y- | <1.1 |
Effective date: 09/19/2005
Guidelines
Ordering & Collection
Specimen Type
Collection
3 mL LAVENDER TOP tube
Pediatric draw: (3) LAVENDER TOP MICROTAINERS
Quantity
requested: entire sample
minimum: 1.5 mL whole blood
Processing
Refrigerate whole blood Stability: EDTA: Refrigerated, 7 days. Ambient, 2 days.
Unacceptable: Other anticoagulants, Frozen specimens.
Performance
LIS Dept Code
Red Cell Disorders (RCD)
Performing Location(s)
| HMC |
Red Cell Disorders
206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Frequency
Monday - Friday
Available STAT?
No