Hepatitis C Qualitative PCR, Tissue
General Information
Lab Name
Hepatitis C Qualitative PCR, Tissue
Lab Code
HCVQLT
Epic Name
Hepatitis C Qualitative PCR, Tissue
Description
The Abbott real-time FDA approved HCV assay detects and quantifies Hepatitis C virus by real-time PCR amplification with primers specific for the 5’UTR region . The RNA concentration detected with a probability of 95% or greater is 12 IU/mL (1.08 log IU/mL).
For Blood samples, see Hepatitis C RNA, Quantitative [HCVQNT].
For HCV genotyping, see Hepatitis C RNA Genotyping by PCR [HCPCGT].
Synonyms
HCV RNA Qualitative, Hepatitis C by PCR, PCR-Hepatitis C
Components
Code | Name |
---|---|
HCVQLS | Hepatitis C Qualitative PCR Specimen Type |
HCVQLR | Hepatitis C Qualitative PCR Result |
HCVQLI | Hepatitis C Qualitative PCR Interpretation |
Interpretation
Method
Real-time Polymerase Chain Reaction.
Reference Range
See individual components
Ordering & Collection
Specimen Type
Collection
Tissue Biopsy: Pea Size in sterile tube/universal transport media.
Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube, No Heparin aspirate in syringe.
Swab: In sterile tube/universal transport media (UTM).
Cellular fluid: 1 mL in sterile tube
Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin samples.
Handling Instructions
Bone Marrow: Send LAVENDER TOP to Laboratory at room temperature within 24 hour of collection.
Note: Separate specimens MUST be submitted when both Viral Culture and PCR testing are ordered.
Quantity
requested: See Collection
minimum: 0.5 mL
Processing
Tissue Biopsy: Freeze at -10°C or colder. Ship on dry ice. Sample may be stored at 2-8°C (ice pack) within 48 hours of collection. Do not freeze/thaw.
Bone Marrow: Ship at room temperature within 24 hours of collection.
Swabs: Freeze at -10°C or colder. Ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Cellular fluid: Freeze at -10°C or colder. Ship frozen samples on dry ice. Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Do not freeze/thaw.
Outside Laboratories:
Buffy coat: Harvest buffy coat from Bone Marrow within 24 hours of collection, Freeze at -10°C or colder.
Tissue/Cellular fluid/Swab: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other |
Virology, Molecular
206-685-6656 Virology Laboratory, Ste 320 |
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Frequency
Wednesday. Results following Thursday.
Available STAT?
No