Laboratory Test Guide

Lupus Anticoagulant, HEXPL

General Information

Lab Name

Lupus Anticoagulant, HEXPL

Lab Code

HEXLA

Epic Name

Lupus Anticoagulant, HEXPL

Description

The Hexagonal Phase Phospholipid assay (HEXLA) is designed for the qualitative detection of Lupus Anticoagulant (LA) in plasma using hexagonal phase phospholipid and LA sensitive APTT reagent. This HEXLA assay is an integrated (screen and confirm) assay for detection of Lupus Anticoagulant. The delta correction of equal to or greater than 6 seconds is determined to be positive for Lupus Inhibitor.

NOTE: This test is performed as part of Lupus Anticoagulant Reflex Panel (LUPI2). HEXLA may be ordered by laboratory staff only.

Synonyms

Hexagonal phospholipid neutralization, HEXPL, Lupus Inhibitor

Interpretation

Method

Clot-based

Reference Range

Units: s

Female Male
AgeRangeAgeRange
0-0.0-5.9 0-0.0-5.9

Effective date: 07/01/2024

Interferences and Limitations

This assay is insensitive to heparin levels up to 2 IU/mL. Dabigatran, direct factor Xa inhibitors (rivaroxaban, apixaban), fondaparinux and warfarin do not interfere with the interpretation of this assay. C-reactive protein/acute phase response does not interfere with the interpretation of this assay.

Ordering & Collection

Specimen Type

Blood

Collection

3 or 5 mL BLUE TOP Tube

Handling Instructions

Laboratory must process the blue top tubes, within 4 hours of blood collection.

Quantity

requested: Whole Blue top tube
minimum: 1.0 mL plasma

Processing

UW-MT: Take Blue top to UW-MT Coag lab for processing. Coag tech will freeze plasma sample for transport to HMC Coag.

HMC: Take Blue top to HMC Coag lab for processing.

Outside Laboratory: Centrifuge Blue tops for 10 minutes, remove plasma & respin plasma for another 10 minutes. Decant and Freeze plasma (minimum 1.0 mL) at -20°C to -80°C. Ship frozen on dry ice.

Performance

LIS Dept Code

Coagulation (COAG)

Performing Location(s)

HMC Coagulation, Special
206-520-4600

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency

Run Monday and Thursday. Reported by end of day.

Available STAT?

No

Billing & Coding

CPT codes

85598

LOINC

96267-0

Interfaced Order Code

UOW5248

Interfaced Result Code

UOW5248