Laboratory Test Guide

HHV6 Qualitative by PCR, Tissue /Cells

General Information

Lab Name

HHV6_PCR Qual, TISS/CELLS

Lab Code

HH6QLT

Epic Name

HHV6 Qualitative PCR, Tissue/Cells

Description

The detection of Human Herpesvirus 6 (HHV-6) by real-time PCR amplification. The primers used in this PCR reaction amplify a fragment of the UL27 and small capsid protein with the same efficiency. Detection of HHV-6 DNA (copies/mL) is achieved by amplifying in each run standard curves consisting of serial dilutions of a known number of DNA containing the HHV-6 amplicons. The minimum virus level that gives a positive result in 95% of replicates is 250 copies/mL (2.40 log copies/mL). Results are reported as “positive” or “not detected”.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF, or Clear non-cellular fluid (e.g. BAL, or Washing), see HHV6 Quantitative by PCR [HH6QN].

Synonyms

Herpes Type 6 by PCR-Tissue or Cells, HHV-6, Human Herpes 6 by PCR-Tissue or Cells

Components

Code	Name
HH6QLS	HHV6 Specimen Descr._PCR Qual
HH6QLR	HHV6 Result_PCR Qual
HH6QLI	HHV6 Interpretation_PCR Qual

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range

See individual components

Ordering & Collection

Specimen Type

Tissue Biopsy, Paraffin Tissue, Bone Marrow, Swab or Cellular fluid (e.g. BAL or Washing)

Collection

Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).

Paraffin Tissue: DO NOT SEND ENTIRE BLOCK and DO NOT SEND SLIDES. Slice 3-4 slices that are 5-50 micron thick in sterile tube.

Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube. No Heparin aspirate in syringe.

Swab: In sterile tube/universal transport media (UTM).

Cellular fluid: 1 mL in sterile tube

Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.

Minimum sample requirement: 0.5 mL

Handling Instructions

Bone Marrow: 5 mL bone marrow in EDTA (LAVENDER TOP) tube to Laboratory at room temperature within 24 hour of collection.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Quantity

requested: See Collection
minimum: 0.5 mL

Processing

Tissue Biopsy: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Paraffin Tissue: Ship at room temperature.

Bone Marrow: Ship at room temperature within 24 hours of collection.

Swabs: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory:

Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder.

Tissue/Cellular fluid/Swab: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code

Virology, Eastlake (EVIR)

Performing Location(s)

Other	Virology, Molecular 206-685-6656 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102