Human Herpes Virus Type 8 by PCR Qualitative Tissue or Cells
General Information
Lab Name
HHV8 PCR Qualitative
Lab Code
HH8QLT
Epic Name
HHV8 PCR Qualitative
Description
The detection of Human Herpesvirus (HHV-8), also known as Kaposi’s sarcoma associated herpesvirus (HHV-8, KSHV), by real-time PCR amplification. The primers used in this PCR reaction amplify the ORF 73 gene. Detection of HHV-8 DNA (copies/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the HHV-8 amplicons. The minimum virus level that gives a positive result in 95% of replicates is 500 copies/mL (2.70 log copies/mL). Results are reported as “positive” or “not detected”.
This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.
For Blood, CSF, or Clear non-cellular fluid (e.g. BAL, or Washing), see HHV8 PCR, Quantitative [HH8QN].
Synonyms
HHV-8, HHV8 PCR Qualitative
Components
| Code | Name |
|---|---|
| HH8QLS | HH8 Qual Specimen Descrip |
| HH8QLR | HH8 Qual Result |
| HH8QLI | HH8 Qual Interp |
Interpretation
Method
Real-time Polymerase Chain Reaction.
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).
Paraffin Tissue: DO NOT SEND ENTIRE BLOCK and DO NOT SEND SLIDES. Slice 3-4 slices that are 5-50 micron thick in sterile tube.
Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube. No Heparin aspirate in syringe.
Swab: In sterile tube/universal transport media (UTM).
Cellular fluid: 1 mL in sterile tube.
Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.
Minimum sample requirement: 0.5 mL
Handling Instructions
Bone Marrow: Send LAVENDER TOP to Laboratory at room temperature within 24 hour of collection.
Note: Separate specimens MUST be submitted when both Viral Culture and PCR testing are ordered.
Quantity
requested: See Collection
minimum: 0.5 mL
Processing
Tissue Biopsy: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Paraffin Tissue: Ship at room temperature.
Bone Marrow: Ship at room temperature within 24 hours of collection.
Swabs: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Outside Laboratory:
Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder and ship on dry ice.
Tissue/Cellular fluid/Swab: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
| Other |
Virology, Molecular
206-685-6656 Virology Laboratory, Ste 320 |
|---|
Frequency
Extraction Wednesday. PCR and results following Thursday.
Available STAT?
No