Heparin Induced Thrombocytopenia Reflexive Panel
General Information
Lab Name
HIT Reflexive Panel
Lab Code
HITRP
Epic Name
HIT Reflexive Panel
Description
Reflex to Serotonin Release Assay (code: RSRAG, with additional billing) ONLY when Heparin Induced Platelet Antibody [code: HIPA] is positive or strong positive.
Assay interference: heparin at a level of >1.0 IU/mL in a test sample will interfere with the HIPA assay and will cause false negative results.
Synonyms
Heparin Induced Antibody, Heparin-PF4 antibody, HIT antibody, HIT Elisa, PF4 Heparin Antibody, Serotonin Release Assay
Components
| Code | Name |
|---|---|
| HITRI | HIT Reflexive Panel Info |
| HIPA | Heparin Induced Platelet Antibody |
| HITOD | Heparin Induced Platelet Optical Density |
| HITRS | SRA Reflex Status |
Interpretation
Reference Range
See individual components
Ordering & Collection
Specimen Type
Collection
Collect both 3 mL BLUE TOP (CITRATE) tube AND 10 mL RED TOP (plain) tube
BLUE TOP Citrated tube needs to be completely filled, the ratio of blood to anticoagulant is critical for valid coagulation results. After the tube has been filled with blood, immediately invert the tube gently several times to prevent coagulation or formation of clots.
Handling Instructions
Lab must process specimens within 6 hours of collection
Quantity
requested: Processed Sample: 1 mL plasma AND 3 mL of Serum
minimum: 0.5 ml Plasma AND 1mL of Serum
Processing
Take BLUE TOP specimen to Coag to process.
Centrifuge RED TOP and separate serum from CLOT within 6 hours. Send serum to UW Coag Lab for refrigeration.
HMC SPS: send HITRP samples to UW Coag Lab using Delivery Express between 9 - 11 am. After 11 am and before noon call LMR to approve sending HITRP as STAT to UWMC-ML.
Outside Laboratories: Centrifuge BLUE Top Tube for 10 minutes, remove plasma and respin plasma for another 10 minutes. Remove plasma within 6 hours of collection.
Freeze plasma (minimum 0.5 mL) @ -20°C to -80°C. Send Frozen on dry ice.
Performance
LIS Dept Code
Coagulation (COAG)
Performing Location(s)
| UW-MT |
Coagulation
206-520-4600 Clinical Lab, Room NW220, |
|---|
Frequency
DailySamples arrive at UW-MT Coagulation lab before or by noon will be run same day.Serotonin Release Assay will be sent out ONLY if HIPA is POS or Strong POS.
Available STAT?
with Lab Medicine Resident approval only