HIV_2 RNA Quantitation

General Information

Lab Name

HIV_2 RNA Quantitation

Lab Code

HIV2VL

Epic Name

HIV-2 RNA Quantitation

Description

The quantitative HIV2VL assay is used to monitor HIV2 RNA in plasma from known HIV-2 infected patients. This RT-PCR assay targets the Long Terminal Repeat (LTR) region of the HIV-2 genome.

Synonyms

HIV 2 RNA, HIV 2 Viral Load

Components

Code Name
H2DES HIV_2 Specimen
H2RSLT HIV_2 RNA Result
H2LOG HIV_2 RNA Copies/mL (Log10)
H2INTP HIV_2 RNA Interpretation

Interpretation

Method

TQPCR

Reference Range

See individual components

Ref. Range Notes

Normal = not detected.

Quantitative range of assay is 10 to 100,000 HIV2 RNA copies/mL plasma

Guidelines

Ordering & Collection

Specimen Type

Blood

Collection

10 mL blood in LAVENDER TOP (EDTA) tube or blood in an 8.5 mL PEARL TOP (EDTA) PPT tube

Handling Instructions

Send sample to lab for processing within 6 hours of collection.

Quantity

requested: 3 to 4 mL EDTA plasma
minimum: 2 mL EDTA plasma

Processing

Lavendar Top tube: Centrifuge. Aliquot 3 to 4 mL plasma into polypropylene tube within 6 hours of collection and freeze at -70°C or colder.

Pearl Top tube: Centrifuge within 6 hours of collection and freeze at -70 °C or colder.

Performance

LIS Dept Code

Virology, HMC (HVIR)

Performing Location(s)

HMC Virology, Retrovirus
206-897-5210

Frequency

Once every 2 weeks; results within 3 weeks

Available STAT?

No

Billing & Coding

CPT codes

87539

LOINC

69354-9

Interfaced Order Code

UOW3855