HIV1 RNA Quantitation
General Information
Lab Name
HIV1 RNA Quantitation
Lab Code
HRTABB
Epic Name
HIV1 Rna Quantitation
Description
The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the FDA approved Roche cobas® HIV-1 quantitative assay for the detection and quantitation of human immunodeficiency virus type 1 in EDTA plasma using real-time PCR amplification.
The range of quantitation of HIV-1 RNA is 20 to 10,000,000 copies/mL (33 to 1.67 x 107 International Units/mL).
The Roche cobas® HIV-1 quantitative assay uses primers specific for the HIV-1 gag gene and the HIV-1 LTR region (dual target).
Synonyms
HIV PCR, HIV Viral Load
Components
| Code | Name |
|---|---|
| HRTDES | HIV RNA Specimen |
| HRRSLT | HIV RNA Result |
| HRLOG | HIV RNA Copies/mL(Log10) |
| HRINTP | HIV RNA Interpretation |
Interpretation
Method
Real-time Polymerase Chain Reaction
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Blood: EDTA plasma or PPT
Unacceptable: plasma from GREEN TOP (Heparin), YELLOW (ACD) or GRAY (Oxylate) and serum (No RED TOP)
Handling Instructions
Freshly drawn whole blood may be stored and/or transported for up to 24 hours at 2°C to 25°C prior to plasma preparation through centrifugation.
Quantity
requested: 2.0 mL plasma
minimum: 0.75 mL plasma
Processing
Blood: Centrifugation upon arrival, remove plasma from cells.
Plasma may be stored and/or transported:
2°C to 8°C for up to 6 days
-18°C ± 2°C for up to 12 weeks
-75°C ± 15°C for long term storage up to 6 months. Avoid multiple freeze/thaw cycles.
Outside Laboratory: Plasma may be stored at 2°C to 8°C for up to 6 days after collection. To ensure testing is completed within this 6-day window, shipment time should not exceed 48 hours from the time of collection, and samples should be shipped on wet ice (ice pack). Alternatively, samples can be frozen at -18°C or colder and shipped on dry ice for extended preservation.
Performance
LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
| CL |
Virology, Renton
206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356 |
206-685-6656 opt 4 |
|---|
Frequency
Tuesday - Saturday. Results same day.
Available STAT?
No