Laboratory Test Guide

Mycoplasma pneumoniae by PCR

General Information

Lab Name

Mycoplasma pneumoniae PCR

Lab Code

MPNPCR

Epic Name

Mycoplasm pneumoniae PCR

Description

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms

Mycoplasma culture, PCR, respiratory

Components

Code	Name
SDES	Specimen Description
SREQ	Special Requests
CULT	Culture
RPT	Report Status

Interpretation

Method

PCR amplified probe

Reference Range

See individual components

Ordering & Collection

Specimen Type

Nasopharynx, Sputum, Bronchial Fluid, Throat swab

Collection

Specimens should be collected into a DNA free container labeled with at least two identifiers. All swabs must be received in Mycoplasma/viral/universal transport media. E-swabs are acceptable. Also accepted: Formalin-fixed paraffin-embedded tissues (FFPE, PET) Unacceptable: Tissues free-floating in formalin; samples containing citrate, or heparin; Blood/Serum specimens

Handling Instructions

Transport to the laboratory immediately or refrigerate overnight.Tissue specimens should be submitted and maintained on Dry Ice.

Quantity

requested: Swab in UTM; 1 mL Fluid; 1 cc Tissue preferred
minimum: Diagnostic yield generally proportional to specimen size.

Processing

Freeze specimens at -20°C upon arrival Outside Laboratory: Refrigerate swabs in transport media for up to 24 hours; freeze at - 20°C for longer periods. Freeze all other specimens in sterile containers at -20°C. Transport all specimens on ice.

Performance

LIS Dept Code

Microbiology (MC)

Performing Location(s)

UW-MT	Microbiology, Molecular Diagnostics 206-520-4600 Clinical Lab, Room # NW177, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday