Laboratory Test Guide

Mycoplasma pneumoniae by PCR

General Information

Lab Name

Mycoplasma pneumoniae PCR

Lab Code

MPNPCR

Epic Name

Mycoplasm pneumoniae PCR

Description

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms

Mycoplasma culture, PCR, respiratory

Components

Code	Name
SDES	Specimen Description
SREQ	Special Requests
CULT	Culture
RPT	Report Status

Interpretation

Method

PCR amplified probe

Reference Range

See individual components

Ordering & Collection

Specimen Type

Nasopharynx, Sputum, Bronchial Fluid, Throat swab

Collection

Specimens should be collected into a DNA free container labeled with at least two identifiers. All swabs must be received in Mycoplasma/viral/universal transport media. E-swabs are acceptable. Also accepted: Formalin-fixed paraffin-embedded tissues (FFPE, PET) Unacceptable: Tissues free-floating in formalin; samples containing citrate, or heparin; Blood/Serum specimens

Handling Instructions

Transport to the laboratory immediately or refrigerate overnight.Tissue specimens should be submitted and maintained on Dry Ice.

Quantity

requested: Swab in UTM; 1 mL Fluid; 1 cc Tissue preferred
minimum: Diagnostic yield generally proportional to specimen size.

Processing

Freeze specimens at -20°C upon arrival Outside Laboratory: Refrigerate swabs in transport media for up to 24 hours; freeze at - 20°C for longer periods. Freeze all other specimens in sterile containers at -20°C. Transport all specimens on ice.

Performance

LIS Dept Code

Microbiology (MC)

Performing Location(s)

UW-MT	Microbiology, Molecular Diagnostics 206-520-4600 ---------------------------------------- Shipping Address Attn: Molecular Microbiology UW CLSPS 1601 Lind Ave SW Room 117 Renton, WA 98057 Phone: 206-520-4600 Alternate phone: 206-598-6147 Performing Lab Address Clinical Microbiology Lab, NW177 University of Washington Medical Center 1959 NE Pacific Street Seattle, WA 98195 Phone: 206-598-5735 Alternate phone: 206-598-6147	Contact Information Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible. email: molmicdx@uw.edu The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT. Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198. For results or other inquiries, we can be reached by phone at the following numbers: Phone: (206) 598-5735 Alternate phone: (206) 598-6147 FAX: (206) 520-4903 For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190

Frequency

Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday