Respiratory MTB Complex PCR

General Information

Lab Name

Respiratory MTB Complex PCR

Lab Code

MTBPC

Epic Name

Respiratory MTB Complex PCR

Description

This test must be ordered with an AFB culture with stain (AFBHSC). Detection of rifampin resistance will be reported if Mycobacterium tuberculosis complex is detected. Exception: outside locations that perform AFB culture may submit sediments of specimens concentrated for AFB culture for testing. This test is reflexively ordered on the first sputum for AFB culture on HMC, UWMC-ML and UWMC-NW patients, except cystic fibrosis patients who have the test performed by request only. Additional tests can be ordered by request as long as the patient has not been on anti-TB medications for longer than 3 days. Reflexive testing is repeated every 28 days on TB negative patients. See also TBCDNA for TB PCR performed at UW-MT Microbiology. For request on FLUIDS or TISSUE see, M. tuberculosis complex DNA Detection [TBCDNA]. Specimen deadlines for same day results are 7 AM @UW-MT and 9 AM @HMC. This test detects M. tuberculosis complex DNA. Specimens are concentrated and cultured (see, AFB Culture with Stain [AFBHSC]).

Synonyms

AFB-Amplified, AMTD, Cepheid, GeneXpert, M. Tuberculosis, MTD, Mycobacteria, PCR-TB, Xpert

Components

Code Name
MTBCPS MTB Complex PCR Specimen
MTBCPR MTB Complex PCR Result

Interpretation

Method

Nucleic Acid Amplification (Cepheid GeneXpert).

Reference Range

See individual components

Guidelines

Ordering & Collection

Specimen Type

Sputum, Bronchoalveolar Lavage (BAL), Bronchial Washings

Collection

Quantity

requested: 5-10 mL Sputum, BAL, or Bronchial Washings, or 1 mL sediment from specimen concentrated for AFB culture
minimum: 2 mL (suboptimal) Sputum, BAL, or Bronchial Washings, or 1 mL sediment from specimen concentrated for AFB culture

Processing

Specimen must be refrigerated within 30 minutes and received within 5 days of collection.

Performance

LIS Dept Code

Serology (SR)

Performing Location(s)

HMC Microbiology
206-744-5858

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency

Monday - Sunday

Available STAT?

No

Billing & Coding

CPT codes

87556

LOINC

85362-2

Interfaced Order Code

UOW5845