Lead & Zinc Protoporph/Heme Ratio
General Information
Lab Name
Pb and ZPPH
Lab Code
PBGRP
Epic Name
Lead Level w/ZPPH
Description
Use: Increased ZPPH levels can be observed in iron deficiency and chronic lead poisoning.
Synonyms
Chronic lead poisoning, PB
Components
| Code | Name |
|---|---|
| ZPPH | Zinc protoporphyrin/Heme Ratio |
| PB | Lead |
| COM9 | Comment |
| PBMTH | Lead Method |
Interpretation
Method
Hematofluorometry
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Preferred: 7 mL blood in ROYAL BLUE (Na2 EDTA) TOP tube.
Also acceptable: LAVENDER or GREEN TOP
Unacceptable: Spun Gel separator tubes
Quantity
requested: entire tube
minimum: 0.5 mL wbld
Processing
If only 1 tube is received, the specimen MUST be at Room Temperature to aliquot for ZPPH. Mix on ROCKER for 5 minutes, then aliquot 0.5 mL whole blood into a plastic vial for ZPPH. Re-stopper the ROYAL BLUE tube for PB (lead) testing.
Refrigerate both whole blood samples after aliquoting.
If the sample is <1.0 mL, it must be shared, with PB testing occurring first at Montlake, and ZPPH testing occurring last at HMC. Rack the sample in the PB rack with a shared specimen slip.
Performance
LIS Dept Code
Chemistry, University (CHU)
Performing Location(s)
| HMC |
Chemistry, Special
206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|
|---|---|---|
| UW-MT |
Chemistry, Special
206-520-4600 Clinical Lab, Room NW220, |
Frequency
PB: Tuesdays, ZPPH: Monday-Friday
Available STAT?
No