Porcine Factor VIII Inhibitor

General Information

Lab Name

Porcine Factor VIII Inhibitor

Lab Code

PF8INH

Epic Name

Porcine Factor VIII Inhibitor

Description

Porcine Factor VIII inhibitor titer is measured to assess the inhibitory activity to recombinant porcine Factor VIII in patients where Obizur® therapy is being considered for the treatment of bleeding. Chromogenic bovine-based Factor VIII reagent is used to measure the residual Factor VIII level and reported in Bethesda Unit.

Synonyms

Porcine F8 Inhibitor, Porcine Factor 8 Inhibitor, Porcine FVIII Inhibitor

Interpretation

Method

Bethesda Assay using chromogenic (2-stage) bovine-based Factor VIII assay

Reference Range

Units: BU

Female Male
AgeRangeAgeRange
0-<0.5 0-<0.5

Effective date: 02/01/2021

Ordering & Collection

Specimen Type

Blood

Collection

3mL or 5mL BLUE TOP (3.2% Citrated) tube

Handling Instructions

The Laboratory MUST process specimen within 4 hours of collection.

Outside Laboratory: Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) at -20° to -80°C. Send frozen on dry ice.

Quantity

requested: Entire sample
minimum: 1.0 mL plasma

Processing

UW-MT Instructions: Take specimen to UW Coagulation Lab for processing. Coagulation tech will freeze plasma sample for transport to HMC Coagulation. If ordered STAT, give to SPS to transport immediately to HMC.

HMC Instructions: Take specimen to Coagulation Lab.

Outside Laboratory: Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) at -20° to -80°C. Send frozen on dry ice.

Performance

LIS Dept Code

Coagulation (COAG)

Performing Location(s)

HMC Coagulation
206-520-4600

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency

Monday-Friday (8 am-3pm). Sample must be at HMC Laboratory by noon to result the same day.

Available STAT?

Yes. Monday-Friday 8am-3pm. For other times, contact Lab Medicine Resident for approval.

Billing & Coding

CPT codes

85335

LOINC

3207-8

Interfaced Order Code

UOW4159

Interfaced Result Code

UOW4159