Laboratory Test Guide

Bordetella pertussis IgG Antibodies (Sendout)

General Information

Lab Name

Bordetella Pertussis IgG Antibody (Sendout)

Lab Code

RBPIGG

Epic Name

Bordetella pertussis IgG Antibody (Sendout)

External Test Id

BORDG

Description

Useful For:

Diagnosis of recent infection with Bordetella pertussis in patients with symptoms consistent with whooping cough for 2 or more weeks
This test should not be used in neonates, young infants or in children between the ages of 4 to 7 years as the routine childhood vaccine schedule may interfere with result interpretation.
This test should not be used as a test of cure, to monitor response to treatment, or to determine vaccine status.

Test Information:

This test may be used to diagnose recent infection with Bordetella pertussis in patients who have not had the acellular pertussis vaccine or booster in the last 6 months.

Synonyms

B. pertussis, B. pertussis Ab, BORDG, Whooping cough

Components

Code	Name
RPERTG	Bordatella pertussis IgG Antibody
RPERTV	Bordatella pertussis IgG Antibody Value

Interpretation

Method

Enzyme-Linked Immunosorbent Assay (ELISA)

Reference Range

See individual components

Ref. Range Notes

Reference Values: <40 IU/mL

Interpretation:

Negative (<40 IU/mL):	No IgG antibodies to pertussis toxin (PT) detected. Results may be falsely negative in patients with less than 2 weeks of symptoms.
Borderline (40-<100 IU/mL):	Recommend follow-up testing in 10 to 14 days if clinically indicated.
Positive (> or =100 IU/mL):	IgG antibodies to pertussis toxin (PT) detected. Results suggest recent infection with or recent vaccination against Bordetella pertussis.

Interferences and Limitations

Cautions:

Immune response following vaccination cannot be distinguished from recent infection.

For diagnosis, clinical symptoms, the patient's age and vaccination history should always be taken into account along with the serological results.

Whooping cough caused by Bordetella parapertussis will not be detected by this assay.

The Centers for Disease Control and Prevention recommend nucleic acid amplification tests (NAAT) or culture as first-line tests for B pertussis infection. However, serologic testing may be useful in patients who are symptomatic for more than 2 weeks.

Guidelines

Washington DOH Notifiable Conditions

Ordering & Collection

Specimen Type

Serum

Collection

Collect 3 mL blood in RED TOP tube or GOLD SST

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred): 30 days; Frozen 30 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, heat-inactivated.

Quantity

requested: 1 mL serum
minimum: 0.5 mL serum

Processing

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Sendouts:

Order Mayo Test: BORDG.
Interfaced: Yes.