Laboratory Test Guide

Factor XIII Qualitative (Sendout)

General Information

Lab Name

Factor XIII Qualitative, Rflx 1:1 Mix (Sendout)

Lab Code

RF13

Epic Name

Factor XIII Qualitative, Rflx 1:1 Mix (Sendout)

External Test Id

3016927

Description

Ordering Recommendation: Most useful if a severe FXIII deficiency is suspected (<1% of normal activity). Distinguishes between FXIII deficiency and a FXIII inhibitor. Abnormal results should be confirmed with quantitative testing. Severe deficiency may be inherited or acquired (typically due to a factor XIII antibody).

Test Algorithm: If Factor 13 Qualitative has a result of "Lysis," then Factor XIII, 1:1 Mix (Sendout) [RF13MX] will be performed at additional charge.

Synonyms

3016927, Clot Solubility, F13, Factor 13, Factor XIII, Fibrin Stabilizing Factor, FX13M, FXIII, Urea Solubility

Interpretation

Method

Qualitative Solubility Assay

Reference Range

Female		Male
Age	Range	Age	Range
0-	No Lysis [NOLYS]	0-	No Lysis [NOLYS]

Effective date: 02/01/2024

Ref. Range Notes

Reference Range:

Factor XIII, Qualitative:

No Lysis within 24 hours

Interferences and Limitations

Cautions: False-positive results (lysis) can be caused by heparin (therapy with unfractionated or low molecular weight heparin or contamination from a line), decreased or abnormal fibrinogen, increased fibrinolysis (inherited or acquired fibrinolytic disorders), fibrinolytic drugs, or other factors that affect clot structure or stability.

Ordering & Collection

Specimen Type

Blood

Collection

5 mL BLUE TOP (sodium citrate) tube

Handling Instructions

Outside Laboratories:

Centrifuge for 10 minutes and remove plasma.
Centrifuge the plasma again for another 10 minutes.
Transfer 2 mL platelet-poor plasma into a separate plastic aliquot (minimum 1 mL). Freeze plasma at -20°C.

Stability: Frozen (required): 2 weeks; Ambient: 4 hours; Refrigerated: Unacceptable.

Note: Critical Frozen. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions: Serum, EDTA plasma, clotted or hemolyzed specimens.

Quantity

requested: 2 mL platelet-poor plasma
minimum: 1 mL platelet-poor plasma

Processing

UW-MT: Immediately forward specimens to Coagulation Lab for processing. Coag: Double-spin the sample and aliquot 2 mL platelet-poor plasma into a separate plastic aliquot (minimum 1 mL). Freeze plasma at -20°C (place in the UW Sendouts frozen rack).

HMC: Immediately forward specimens to Coagulation Lab for processing. Coag: Double-spin the sample and aliquot 2 mL platelet-poor plasma into a separate plastic aliquot (minimum 1 mL). Freeze plasma at -20°C. Forward to UW-MT for sendout.

UW-NW or Fred Hutch: Centrifuge for 10 minutes and remove plasma. Centrifuge the plasma again for another 10 minutes. Transfer 2 mL platelet-poor plasma into a separate plastic aliquot (minimum 1 mL). Freeze plasma at -20°C. Forward to UW-MT for sendout.

Sendouts:

Order ARUP Test: 3016927.
Interfaced: Yes. [Interface: 602; Department: ARPZ]

Stability: Frozen (required): 2 weeks; Ambient: 4 hours; Refrigerated: Unacceptable.

Note: Critical Frozen. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions: Serum, EDTA plasma, clotted or hemolyzed specimens.

Performance

LIS Dept Code

Sendouts ARUP Lab (FZ) (ARPZ)

Performing Location(s)

Sendout	ARUP Laboratories, Inc. 800-522-2787 500 Chipeta Way Salt Lake City, UT 84108

Frequency

Performed: Sunday through Saturday. Reported: 2-3 days from sample receipt at ARUP.