Plt Autoantibody Test (Sendout)
General Information
Lab Name
Plt Autoantibody Test (Sendout)
Lab Code
RPAA
Epic Name
Platelet Ab Screen Auto Test (Sendout)
External Test Id
3640-00
Description
Test Information: Idiopathic Thrombocytopenic Purpura (ITP) is a diagnosis associated with elevated platelet associated IgG, IgM or both. Positive autoantibody test results are indicative of autoimmune destruction. Positive indirect antibody test results, using patient’s plasma against normal random platelets, may indicate ITP depending upon the patient’s transfusion or pregnancy history. In approximately 85% of patients with ITP, the presence of an autoantibody can be detected.
Additional Test Information: Patients who require autoantibody testing must be platelet transfusion independent for approximately 5 days to ensure that the platelets being tested are the patient’s own platelets and not circulating donor platelets.
Synonyms
3640-00, Idiopathic Thrombocytopenic Purpura, ITP Workup, Platelet Flow Cytometry
Components
| Code | Name |
|---|---|
| RPAAR | Plt Autoantibody Result (Sendout) |
| S2PAA | Plt Autoantibody Testing Lab |
Interpretation
Method
Flow Cytometry
Reference Range
See individual components
Ordering & Collection
Specimen Type
Collection
5 mL blood in BLUE TOP (Citrate) AND appropriate amount of blood in LAVENDER TOP tube based on platelet count, see below.
- Platelet Count: >= 50,000, Collect: 2 x 10 mL EDTA/LAVENDER tubes
- Platelet Count: 20,000 to 49,999, Collect: 3 x 10 mL EDTA/LAVENDER tubes
- Platelet Count: 10,000 to 20,000, Collect: 4 x 10 mL EDTA/LAVENDER tubes
- Platelet Count: Less than 10,000, Collect: DO NOT DRAW***
- ***Cannot recover sufficient platelets to perform a direct assay of antibody bound to autologous platelets. Contact Lab Supervisor for testing options at (206) 689-6542.
Forms & Requisitions
Bloodworks NW Platelet Immunology Laboratory Requisition
Handling Instructions
Outside Laboratories: Maintain and transport whole blood samples at ambient temperature. Expedite transport to the laboratory. Samples must be received in the Platelet Immunology Lab within 24 hours of collection.
Note: Samples received in the Platelet Immunology Lab after 10:00 am on Friday or over the weekend will need to be re-collected.
Quantity
requested: Refer to collection information.
minimum: Refer to collection information.
Processing
Send samples at room temperature with a completed Bloodworks Northwest requisition or copy of the Epic requisition.
- Samples must be received by BloodWorks NW Platelet Immunology lab within 24 hours of collection.
- Samples sent on Friday must be received in lab by 10:00 am.
Performance
LIS Dept Code
Sendouts (SO)
Performing Location(s)
| Sendout |
Bloodworks Northwest
206-292-6500 921 Terry Ave |
|---|
Other Locations/Notes
Bloodworks Northwest Platelet Immunology Lab: (206) 689-6543
Frequency
Performed: Monday - Friday morning. TAT: 1 business day.
Available STAT?
No