1,25 Dihydroxy Vitamin D
General Information
Lab Name
1,25 Dihydroxy Vitamin D
Lab Code
VTD125
Epic Name
1,25 Dihydroxy Vitamin D
Description
The concentration of 1,25(OH)2D is determined by immunoaffinity extraction followed by liquid chromatography-tandem mass spectrometry. The reportable range is 5-200 pg/mL for both 1,25(OH)2D2 and 1,25(OH)2D3, which are summed to give the total 1,25(OH)2D concentration, with imprecision between 8.7 and 13.0 %CV.
Synonyms
Dihydroxyvitamin D
Components
| Code | Name |
|---|---|
| 125VD2 | 1,25 Dihydroxy Vitamin D2 |
| 125VD3 | 1,25 Dihydroxy Vitamin D3 |
| 125VDT | 1,25 Dihydroxy Vitamin D Total |
| 125VDM | 1,25 Dihydroxy Vitamin D Method |
Interpretation
Method
Serum immunoaffinity extraction. Isotope dilution liquid chromatography mass spectrometry
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
All Locations:
Preferred: 4 mL blood in GOLD SST tube
Also Acceptable: 4 mL blood in ORANGE RST or RED TOP tube
Pediatric: 2 Full GOLD MICROTAINER
Handling Instructions
Testing is performed once weekly on Wednesday morning. Samples must be received by the performing lab by 5:00 a.m in order to be included in the weekly run. It is recommended that samples be collected prior to Wednesday morning in order to ensure that samples are included in the weekly run.
Quantity
requested: 1 mL serum
minimum: 0.8 mL serum
Processing
Freeze serum at -20°C
Performance
LIS Dept Code
Chemistry, University (CHU)
Performing Location(s)
| UW-MT |
Chemistry, General
206-520-4600 Clinical Lab, Room NW220, |
|---|
Frequency
Monday, results next day
Available STAT?
No