Von Willebrand Factor Multimer Analysis
General Information
Lab Name
VWF Multimer Analysis
Lab Code
VWMULT
Epic Name
Von Willebrand Factor Multimer Analysis
Description
von Willebrand Factor (VWF) multimer proteins are separated according to their molecular weight by agarose gel electrophoresis, subjected to immunofixation with anti-VWF antiserum and visualized using peroxidase-labeled antibody.
The electrophoretic patterns of high, intermediate and low molecular weight multimers are analyzed and used in conjunction with other von Willebrand disease (VWD) tests (Factor 8 activity, VWF antigen and activity and collagen binding) to type/subtype VWD. The multimer test is also useful in suspected cases of acquired VWD and when results of other vWD tests (Factor VIII, VWF Antigen and Activity, VWF collagen binding) are abnormally low or discordant.
Synonyms
VWF Multimer
Components
| Code | Name |
|---|---|
| MULTI | VWF Multimer Analysis |
| MULTIM | VWF Multimer Method |
Interpretation
Method
Agarose gel electrophoresis with immunofixation
Reference Range
See individual components
Ref. Range Notes
High, Intermediate and Low Molecular Weight Multimers Present
Ordering & Collection
Specimen Type
Collection
3 or 5 mL BLUE (CITRATE) tube
Handling Instructions
The Laboratory MUST process specimen within 4 hrs of blood collection.
Quantity
requested: entire sample
Processing
UW-MT Instructions: Take specimen to UW-MT Coag lab for processing. Coag tech will freeze plasma sample for transport to HMC Coag.
HMC Instructions: Take specimen to HMC Coag lab for processing.
Outside Laboratory: Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) @ -20°C to -80°C. Send Frozen on dry ice.
Performance
LIS Dept Code
Coagulation (COAG)
Performing Location(s)
| HMC |
Coagulation
206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Frequency
Run on Wednesday and result entered by end of day Thursday.
Available STAT?
No