Pathology Presents: Lighting up native biomolecules with stimulated Raman Scattering microscopy
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Daniel Fu, PhD
Department of Chemistry
University of Washington
Optical microscopy plays a pivotal role in studying the pathophysiology of tissue. In the past two decades, major advances have been made in transcending the sensitivity, spatial resolution, and temporal resolution limit, especially with the development of various forms of advanced fluorescence microscopy and a large repertoire of fluorescent labels. However, fluorescent labeling has limitations in specificity, preserving function, and photostability. In this talk, I will introduce a new optical imaging technique called stimulated Raman scattering (SRS) microscopy. It uses intrinsic vibrational signatures of molecules to probe their spatiotemporal distributions in cells, tissues, and organisms. Many native biomolecules as well as small drug molecules can be directly imaged by SRS without labeling, thereby opening up new opportunities to study tissue pathophysiology. I will present our recent efforts in developing and applying SRS microscopy to image chemical composition of tissue and study the metabolic dynamics of small molecules.