Laboratory Test Guide

Chagas Disease Molecular Detection (Sendout)

General Information

Lab Name

Lab Code

1061

External Test Id

CDC-10475

Description

Test Information: This assay is used in clinically indicated situations such as acute or congenital infections, post-transplant from a donor with confirmed T. cruzi infection, or risk of reactivation due to immunocompromise in patients with chronic Chagas disease. Serological testing is the preferred method to diagnose chronic infection in patients.

Ordering Requirements: A completed Washington State PHL Serology Requisition (or CDC 50.34 Specimen Submission Form, if sent directly) must accompany all submissions. Providers should coordinate with the LHJ epidemiologists or with the appropriate CDC lab contacts (if sending direct).

Seattle King Co. Public Health Epidemiologist: 206-296-4774
WA PHL Epidemiology (Main): 206-418-5500
CDC Chagas Disease Molecular Detection test guide (refer to "CDC Pre-Approval" section for points of contact).

Approval: Lab Medicine Resident or Microbiology Fellow approval required. Testing must be coordinated with LHJ epidemiologists or directly with the CDC.

Synonyms

American trypanosomiasis, CDC-10475, Chagas PCR, Trypanosoma cruzi

Interpretation

Method

Real-time Polymerase Chain Reaction (PCR)

Guidelines

Washington DOH Notifiable Conditions

Ordering & Collection

Specimen Type

Blood

Collection

Collect 5 mL blood in LAVENDER TOP (EDTA) tube

Also Accepted: PINK EDTA

Forms & Requisitions

CDC Form 50.34
WPHL Serology/Virology/HIV Requisition

Approval Required

Lab Medicine Resident or Microbiology Fellow approval required. Testing must be coordinated with state lab epidemiologists or pre-approved by CDC.

Quantity

requested: 5 mL EDTA whole blood
minimum: 2.2 mL EDTA whole blood (Infants <1 year old: 0.3 mL whole blood)

Processing

Refrigerate whole blood in original collection container.

Login: SPMSND-;REFRIG

MSNDTO: CDC
MSPTYP: WB
MTSTRQ: ;Chagas Disease Molecular Detection (CDC Test 10475)

Sendouts: Samples may be sent to WPHL or directly to the CDC, depending on instruction from the LMR/Micro Fellow.

To CDC via Washington State Public Health Lab:

Provider must complete the WPHL Serology Requisition. Add the CAST fax number to the form to ensure proper result routing.
Send sample with the DLMP courier or Delivery Express courier.

Direct Submission to CDC:

CDC Test: CDC-10475.
To prevent sample rejections, review the CDC requisition for completion prior to shipment.
- There must be two matching patient identifiers present on the sample and on the CDC requisition. One identifier must be the name, the other identifier can be the MRN, specimen ID, or date of birth. Note that because Sunquest CID labels do not contain the DOB, then either or the MRN or specimen ID must be included on the requisition or the date of birth added to the specimen label.
- Requisitions that are pre-filled by the provider may require additional information to be added after the sample is collected. This may include: collection date, sample type, date sent to CDC, specimen ID number, etc. Per CDC requirements, additional information may be written on an electronically completed form as long as it is initialed by lab staff making the correction/addition.
- CAST must be entered as the "intermediate submitter" to ensure that result reports are properly routed to the lab from the WAPHL.
Send sample refrigerated (2-8°C) Monday - Thursday only via FedEx Priority Overnight.
- Samples must be received within 7 days of collection.

Stability: Refrigerated (required): 7 days; Frozen: Unacceptable; Ambient: Unacceptable.

Performance

LIS Dept Code

Performing Location(s)

Sendout	Centers for Disease Control and Prevention 855-612-7575 1600 Clifton Road NE Atlanta, GA 30333