Laboratory Test Guide

Histoplasma and Blastomyces Antigen, Urine (Sendout)

General Information

Lab Name

Lab Code

1112

External Test Id

UHBAG

Description

Useful For:

Diagnosing Histoplasma capsulatum or Blastomyces dermatitidis infection without differentiation between the organisms
Monitor antigen levels following initiation of antifungal treatment

Synonyms

RHSTOU, UHBAG

Interpretation

Method

Enzyme Immunoassay (EIA)

Ref. Range Notes

Reference Values:

Histoplasma/Blastomyces Antigen Result:	Not Detected
Histoplasma/Blastomyces Antigen Value:	Not Detected (0.0 ng/mL)

Interferences and Limitations

Cautions:

Due to significant cross-reactivity between galactomannan antigens from Blastomyces and Histoplasma,
this assay does not differentiate between these 2 dimorphic fungal agents. To differentiate, consider fungal
culture, molecular testing, or serology testing.
Positive results should be correlated with other clinical and laboratory findings (eg, culture, serology).
Low-level positive antigen levels may persist following resolution of infection and completion of appropriate
treatment regimen.
Sensitivity of this assay to detect antigen from species other than Blastomyces dermatitidis or Histoplasma
capsulatum is unknown.
False-positive results may occur less frequently with other dimorphic agents (eg, Coccidioides).

Ordering & Collection

Specimen Type

Urine

Collection

Collect random urine in a preservative-free urine collection container.

Handling Instructions

Outside Laboratories:

Transfer 3 mL urine (2.5 mL min) to a separate screw-cap vial. Do not centrifuge to remove particulates.
Refrigerate sample while awaiting shipment. Transport with a cold pack.

Stability: Refrigerated (preferred): 14 days; Frozen: 28 days; Ambient: 7 days

Quantity

requested: 3 mL urine
minimum: 2.5 mL urine

Processing

Transfer 3 mL urine (2.5 mL min) to a separate screw-cap vial. Do not centrifuge to remove particulates. Refrigerate urine.

RSTYP1: URN
RSNDT1: MAYOSD
RSTYP1: ;Histoplasma and Blastomyces Antigen, Urine (Mayo Test UHBAG)

Sendouts:

Order Mayo Test: UHBAG

Stability: Refrigerated (preferred): 14 days; Frozen: 28 days; Ambient: 7 days.

Performance

LIS Dept Code

Performing Location(s)

Sendout	Mayo Clinic Laboratories (Superior Drive) 800-533-1710 3050 Superior Drive NW Rochester, MN 55901

Frequency

Performed: Monday through Sunday. Reported: 1-3 days from sample receipt at Mayo Clinic Laboratories.