Laboratory Test Guide

Neoplasia IFISH [UWMC Cytogenetics and Genomics]

General Information

Lab Name

Lab Code

423

Description

This test uses a variety of FISH probes to detect specific rearrangements associated with neoplastic disorders, including both balanced (translocations and inversions) and imbalanced (aneuploidy, deletions, duplications, or amplification) rearrangements. Testing is performed by UWMC - Montlake DLMP Cytogenetics and Genomics Laboratories.

EPIC Ordering:

Test Name: FISH, Neoplasia
Order Code: LAB015
For orders submitted electronically via Epic, a separate requisition form is not required.

Synonyms

13q deletion, 20q deletion, 4q12 deletion, 4q12 rearrangement, 5q deletion, 5q33 rearrangement, 7q deletion, ABL1, Acute Lymphocytic Leukemia, Acute Myeloid Leukemia, Acute Promyelocytic Leukemia, ALK, ALL, AML, Amyloidosis, Anemia, APL, ASS1, ATM, B-cell lymphoma, BCL2, BCL6, BCR, BCR/ABL1, BIRC3, Blast Crisis, Bleeding Disorder, Burkitt's Lymphoma, Carcinoma, CBFB, CCND1, CDKN2A, CEN12, CEN15, CEN17, CEN7, CEN8, CEN9, CHIC2, Chronic Lymphocytic Leukemia, Chronic Myelogenous Leukemia, Chronic Myelomonocytic Leukemia, CLL, CML, CMML, D13S319, D20S108, D5S23, D5S721, D7S486, Diffuse Large B-Cell Lymphoma, EGFR, EGR1, Elevated Platelets, Eosinophilia, ETV6, EVI1, EWSR1, Fanconi Anemia, FGFR1, FGFR3, FIP1L1, FISH, Fluorescence In Situ Hybridization, Follicular Lymphoma, FOXO1, Hairy Cell Leukemia, Hemangioma, Hemolysis, HES, Hodgkin Lymphoma, Hypereosinophilic syndrome, Idiopathic Thrombocytopenic Purpura, IGH, inv(16), inv(3), KIT, LAB015, Leukemia, Lipoma, Liposarcoma, LNX, Lymphoma, Lymphoproliferative Disorder, Macrocytoma, Macroglobulinemia, MAF, MALT, MALT1, Mantle Cell Lymphoma, Marginal Cell Lymphoma, Mastocytosis, MDS, Melanoma, MLL, MM, Monoclonal Gammopathy, Monosomy, MPD, Multiple Myeloma, MYB, MYC, Mycosis Fungoides, Myelodysplastic Syndrome, Myeloma, Myeloproliferative Neoplasm, Neoplasia, Neuroblastoma, Night Sweats, Non-Hodgkin Lymphoma, Pancytopenia, PDGFRA, PDGFRB, Plasmacytoma, PML, Polycythemia, RARA, Refractory Anemia, ROS1, RUNX1, RUNX1T1, Sarcoma, SCFD2, SS18, Systemic Mast Cell Disease, t(11;14), t(14;18), t(15;17), t(8;21), t(9;22), T-Cell Leukemia, T-Cell Lymphoma, TCL1A, THOR, Thrombocytopenia, TP53, TRA, TRB, TRD, Treatment-Related Leukemia, Waldenstrom's Macroglobulinemia

Interpretation

Guidelines

Thoracic Oncology Reflex (THOR) Panel

Ordering & Collection

Specimen Type

Bone Core, Bone Marrow, Cerebral Spinal Fluid, FFPE Tissue, Formalin Fixed Paraffin Embedded Tissue, Leukemic Peripheral Blood, Lymph Node, Paracentesis Fluid, Solid Tumor

Collection

Bone marrow:

Collect up to 5ml in preservative-free sodium heparin (green top vacutainer) OR in a sterile heparinized syringe.

Bone core or lymph node:

Immediately place in a sterile vial with 3-5ml tissue transport media (supplied by the lab - call 206.598.4488).

Leukemic Peripheral Blood:

Collect 10ml of blood in preservative-free sodium heparin (green top vacutainer) OR in a sterile heparinized syringe.

Paracentesis Fluid or Cerebrospinal Fluid:

Collect in a sterile vial.

Solid Tumor (fresh):

Obtain sample (0.15-2.0cm3) under sterile conditions and place in transport media (supplied by the lab - call 206.598.4488).
Keep refrigerated.

Solid Fumor (FFPE/Formalin-Fixed, Paraffin-Embedded):

Provide an unstained slide (4μm thick section) per probe of IFISH and a corresponding H&E stained slide.
Do not send specimens that have been exposed to decalcifying agents.
Mail slides in a protected mailing container.

Forms & Requisitions

Cytogenetics Neoplasia Test Request Form

Neoplasia IFISH Supplemental Request Form

Handling Instructions

Outside Laboratories:

Maintain and transport samples at ambient temperature unless otherwise indicated in the Collection section.
A completed request form must accompany all orders.
Specimens are accepted 24 hrs/day, 7 days/week.
Ship to:
- Cytogenetics and Genomics Laboratory
  University of Washington Medical Center, Room NW-125
  1959 NE Pacific Street
  Seattle, WA 98195-6100

Quantity

requested: Varies; refer to Collection information.
minimum: Varies; refer to Collection information.

Processing

Hold specimen(s) at room temperature.

Call Cytogenetics and Genomics Laboratory at (206) 598-4489 for pick up. Cytogenetics is staffed on weekends to process specimens.
If no answer or after hours, call (206) 598-4488 to leave message.

Performance

LIS Dept Code

Performing Location(s)

Pathology	Cytogenetics Laboratory 206-598-4488

Other Locations/Notes

Cytogenetics and Genomics Laboratory

University of Washington Medical Center, Room NW-125
1959 NE Pacific Street
Seattle, WA 98195-6100

Ph: (206) 598-4488
Fax: (206) 598-2610
Email: cytogene@uw.edu

Frequency

Performed daily. TAT for fresh samples: 1-6 days. TAT for FFPE samples: 2-8 days.