Anti Neutrophil Cytoplasm Panel
General Information
Lab Name
Anti Neutrophil Cytoplasm Panel
Lab Code
ANCAP
Epic Name
Anti Neutrophil Cytoplasm Panel
Description
For neutrophil surface antibodies, see information under Lab Mnemonic: 102.
Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.
Synonyms
ANCA-Panel, ANCAG, Atypical ANCA, C ANCA, MPO, P ANCA, PR3, Vasculitis, Wegener's Antigen
Components
| Code | Name |
|---|---|
| ANCAIF | Antineutrophil Cytoplasmic Antibody by IF |
| APR3L | Anti PR3 Level |
| APR3C | Anti PR3 Comment |
| AMPOL | Anti MPO Level |
| AMPOC | Anti MPO Comment |
Interpretation
Method
Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.
Reference Range
See individual components
Ref. Range Notes
Established by testing healthy blood donors during method validation.
Interferences and Limitations
Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.
Guidelines
Ordering & Collection
Specimen Type
Collection
6 mL blood in Red Top or GOLD SST tube
Handling Instructions
Note: If requested STAT the ordering physician MUST call the Laboratory Medicine Resident on-call for approval.
Quantity
requested: 2 mL serum
minimum: 1 mL serum
Processing
Separate serum and refrigerate. Stable 14 days refrigerated. Stable long term frozen.
Performance
LIS Dept Code
Immunology (IMM)
Performing Location(s)
| UW-MT |
Immunology
206-520-4600 Clinical Lab, Room NW220, |
|---|
Frequency
Monday-Friday
Available STAT?
Lab Medicine Resident approval required.