Activated Protein C Resistance
General Information
Lab Name
Activated Protein C Resistance
Lab Code
APCR
Epic Name
Activated Protein C Resist.
Description
The Activated Protein C Resistance (APCR) is a plasma based functional assay used to screen for the factor V Leiden mutation (F5:p.R506Q). Patients with factor V Leiden mutation have increased risk for incidental or recurrent venous thromboembolism.
Synonyms
APC Resistance, Factor 5 Leiden Protein Assay, Factor V Leiden Protein Assay, Protein C Cofactor
Interpretation
Method
Clot based
Reference Range
| Female | Male | ||
|---|---|---|---|
| Age | Range | Age | Range |
| 0- | Negative [NRN] | 0- | Negative [NRN] |
Effective date: 04/01/2010
Interferences and Limitations
Patients on Aprotinin, Protamine or direct thrombin inhibitors such as hirudin (Refludan), bivalidrudin (Angiomax), Dabigatran (Pradaxa), and argatroban (Novastan) may interfere with this assay. If patients were administered with Aprotinin, Protamine or direct thrombin inhibitors, it is recommended to wait at least 24 hours before collecting samples or order F5DNA assay.
Ordering & Collection
Specimen Type
Collection
3 or 5 mL BLUE (CITRATE) tube
Forms & Requisitions
Outside clients should fill and submit Coagulation Patient Clinical History Form: Coagulation Patient Clinical History Form
Approval Required
**Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.**
Handling Instructions
The Laboratory MUST process specimen within 4 hours of blood collection.
Quantity
requested: entire sample
minimum: 1.0 mL plasma
Processing
**Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.** Approval is NOT required for hospital outpatients, clinic patients or outside clients.
UW-MT Instructions: Take specimen to UW-MT Coag for processing. Coag tech will freeze plasma sample for transport to HMC Coag."
HMC Instructions: Take specimen to HMC Coag bench for processing.
Outside Laboratory:Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) @ -20°C to -80°C. Send Frozen on dry ice.
Performance
LIS Dept Code
Coagulation (COAG)
Performing Location(s)
| HMC |
Coagulation
206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Frequency
Run Monday and Thursday. Reported by end of day.
Available STAT?
No