AFB ID by Sequence Analysis

General Information

Lab Name

AFB ID by Sequence Analysis

Lab Code

ASEQID

Epic Name

AFB ID by Sequence Analysis

Description

Culture that is identified as Mycobacterium tuberculosis (MTB), MTB complex, or M. bovis will have susceptibilities reflexively ordered for an additional charge. Blood bottles and trek broth must have organisms visible by stain to be acceptable for sequence analysis.

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms

Acid fast bacilli PCR, acid fast bacteria, acid-fast bacteria, AFB identification, AFB PCR, AFB PCR from pure culture, AFB sequencing, bacteria 16S, Mycobacteria PCR, Mycobacterial sequencing, Mycobacterial identification of isolated colonies, Mycobacterium identification

Components

Code Name
ASQID AFB Sequence: Identification
ASQSI AFB Sequence: Specimen Description
ASQSR AFB Sequence: Special Requests
ASQSC AFB Sequence: Specimen Comments
ASQREV AFB Sequence: Pathologist Review
ASQME AFB Sequence: Method Note

Interpretation

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range

See individual components

Guidelines

Ordering & Collection

Specimen Type

Pure Culture

Collection

Handling Instructions

Send pure culture (plate, tube or positive blood culture bottle) at room or refrigerated temperature. Follow approved regulations for transporting infectious substances. A mixed culture will be subject to an additional charge.

Quantity

requested: Plate, slant or bottle

Processing

Performance

LIS Dept Code

Micro Molecular Diag (MMD)

Performing Location(s)

UW-MT Microbiology, Molecular Diagnostics
206-520-4600

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Shipping Address

Attn: Molecular Microbiology
UW CLSPS
1601 Lind Ave SW Room 117
Renton, WA 98057
Phone: 206-520-4600
Alternate phone: 206-598-6147

Performing Lab Address

Clinical Microbiology Lab, NW177
University of Washington Medical Center
1959 NE Pacific Street
Seattle, WA 98195
Phone: 206-598-5735
Alternate phone: 206-598-6147

Contact Information

Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible.

email: molmicdx@uw.edu

The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT.

Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198.

For results or other inquiries, we can be reached by phone at the following numbers:

  • Phone: (206) 598-5735
  • Alternate phone: (206) 598-6147
  • FAX: (206) 520-4903

For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190

Frequency

Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.

Available STAT?

No

Billing & Coding

CPT codes

87153

LOINC

14974-0

Interfaced Order Code

UOW4474