AFB ID by Sequence Analysis
General Information
Lab Name
AFB ID by Sequence Analysis
Lab Code
ASEQID
Epic Name
AFB ID by Sequence Analysis
Description
Culture that is identified as Mycobacterium tuberculosis (MTB), MTB complex, or M. bovis will have susceptibilities reflexively ordered for an additional charge. Blood bottles and trek broth must have organisms visible by stain to be acceptable for sequence analysis.
For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website
Synonyms
Acid fast bacilli PCR, acid fast bacteria, acid-fast bacteria, AFB identification, AFB PCR, AFB PCR from pure culture, AFB sequencing, bacteria 16S, Mycobacteria PCR, Mycobacterial sequencing, Mycobacterial identification of isolated colonies, Mycobacterium identification
Components
| Code | Name |
|---|---|
| ASQID | AFB Sequence: Identification |
| ASQSI | AFB Sequence: Specimen Description |
| ASQSR | AFB Sequence: Special Requests |
| ASQSC | AFB Sequence: Specimen Comments |
| ASQREV | AFB Sequence: Pathologist Review |
| ASQME | AFB Sequence: Method Note |
Interpretation
Method
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Handling Instructions
Send pure culture (plate, tube or positive blood culture bottle) at room or refrigerated temperature. Follow approved regulations for transporting infectious substances. A mixed culture will be subject to an additional charge.
Quantity
requested: Plate, slant or bottle
Processing
Performance
LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
| UW-MT |
Microbiology, Molecular Diagnostics
206-520-4600 Clinical Lab, Room # NW177, |
|---|
Frequency
Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.
Available STAT?
No