Broad-Range PCRs
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Acceptable Specimens
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Bacteria
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Acceptable
- Fresh frozen tissue
- Fresh frozen fluid. Common examples include: cerebrospinal fluid, pleural fluid, pericardial fluid, urine, bronchial lavage, joint fluid, bone marrow, vitreous fluid, etc.
- Formalin Fixed Paraffin-embedded tissues (FFPE, PET): blocks, scrolls, and unstained slides
- eSwabs, UTM (universal transport media), body fluid/bone marrow in EDTA (not including blood)
- Sodium polyanethol sulfonate (SPS, Wampole Isolator Tubes) acceptable with disclaimer
- Respiratory and genitourinary samples may be submitted if Bacterial PCR reflex NGS [BCTDNA] is selected
Unacceptable
- Sputum
- Blood, serum, plasma
- Stool/rectal swabs
- Citrated or heparinized solutions
- Tissues floating in formalin
- Swab/fluid collected in tube containing agar
- Specimens treated with strong acid Decalcifying/Chelating agents such as Hydrochloric acid and formic acid
- Specimens incubated above room temperature
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Fungi
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Acceptable
- Fresh frozen tissue
- Skin scrapings
- Fresh frozen fluid. Common examples include: cerebrospinal fluid, pleural fluid, pericardial fluid, urine, bronchial lavage, joint fluid, bone marrow, vitreous fluid, etc.
- Formalin Fixed Paraffin-embedded tissues (FFPE, PET): blocks, scrolls, and unstained slides
- Sodium polyanethol sulfonate (SPS, Wampole Isolator Tubes) acceptable with disclaimer
- eSwabs*, UTM (universal transport media), body fluid/bone marrow in EDTA (not including blood)
*Fungal PCR may have interference due to some lots of eSwabs which have been found to contain Saccharomyces cerevisiae DNA, resulting in false positive detection. Clinical correlation and/or retesting with a different collection method is advised. The detection of S. cerevisiae from eSwab specimens can interfere with our ability to rule out other fungal DNA.
Unacceptable
- Sputum
- Upper respiratory specimens
- Blood, serum, plasma
- Stool/rectal swabs
- Genitourinary specimens
- Citrated or heparinized solutions
- Tissues floating in formalin
- Swab/fluid collected in tube containing agar
- Specimens treated with strong acid Decalcifying/Chelating agents such as Hydrochloric acid and formic acid
- Specimens collected in Thioglycolate medium
- Specimens incubated above room temperature
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AFB
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Acceptable
- Fresh frozen tissue
- Fresh frozen fluid. Common examples include: cerebrospinal fluid, pleural fluid, pericardial fluid, urine, bronchial lavage, joint fluid, bone marrow, vitreous fluid, etc.
- Formalin Fixed Paraffin-embedded tissues (FFPE, PET): blocks, scrolls, and unstained slides
- Sodium polyanethol sulfonate (SPS, Wampole Isolator Tubes) acceptable with disclaimer
- eSwabs, UTM (universal transport media), body fluid/bone marrow in EDTA (not including blood)
Unacceptable
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