Parvovirus B19 by PCR, Quantitative

General Information

Lab Name

Parvovirus B19 Quantitative PCR, Blood/CSF

Lab Code

B19PCQ

Epic Name

Parvovirus B19 Quantitative PCR, Blood/CSF

Description

The detection and quantitation of Parvovirus B19 by real-time PCR amplification. The primers used in this PCR reaction amplify the non-structural protein region. Quantitation of B19 (IU/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the B19 amplicons. The limit of detection (LoD), defined as the minimum viral level detectable in 95% of replicates, and the lower limit of quantification (LLoQ) are both 2000 IU/mL (3.30 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For tissue biopsy, bone marrow, or cellular/viscous fluids (e.g., BAL or washings), refer to Parvovirus B19 by PCR Qualitative Tissue or Cells [B19QLT].

Synonyms

B19 Parvovirus, Human B19 Virus

Components

Code	Name
SPDS23	Parvovirus B19 Specimen
B19PRE	Parvovirus B19 Preliminary Result
B19QNR	Parvovirus B19 DNA Quantitative Result
B19LOG	Parvovirus B19 DNA IU/mL (Log 10)
IN95	Parvovirus B19 Quantitative Interpretation

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range

See individual components

Guidelines

Specialty Testing in UW Virology Laboratory

Ordering & Collection

Specimen Type

Blood, CSF, Clear non-cellular fluid (e.g. BAL, or Washing)

Collection

Blood: 3 mL blood in EDTA (LAVENDER TOP) tube.

CSF: 1 mL in sterile container.

Clear non-cellular fluid (e.g. BAL or Washing): 1 mL in sterile container.

Also Acceptable: 3 mL blood in GOLD SST, RED, or PPT (PEARL TOP) tube.

Unacceptable samples: Plasma collected in sodium or lithium heparin.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Minimum sample requirement: 0.5 mL

Quantity

requested: 1 mL
minimum: 0.5 mL

Processing

Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

CSF: If CSF has cellular material, centrifuge & aliquot clear CSF. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Clear non-cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code

Central Lab Virology (RVIR)

Performing Location(s)

CL	Division of Infectious Disease Diagnostics (DIDD) - Virology 1601 Lind Ave SW Renton, WA 98057	Phone: 206-685-6656 option 8 Hours of Operation: Mon–Fri: 7:00 AM–7:00 PM Sat: 9:00 AM–4:00 PM

Laboratory Test Guide