Parvovirus B19 by PCR Qualitative Tissue or Cells
General Information
Lab Name
Parvovirus B19 Qualitative PCR, Tissue/Cells
Lab Code
B19QLT
Epic Name
Parvovirus B19 Qualitative PCR, Tissue/Cells
Description
The detection of parvovirus B19 by real-time PCR amplification. The primers used in this PCR reaction amplify the non-structural protein region. Detection of B19 (IU/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the B19 amplicons. The limit of detection (LoD), defined as the minimum viral level detectable in 95% of replicates is 2000 IU/mL (3.30 log IU/mL).
This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.
For Blood, CSF, or Clear non-cellular fluid (e.g. BAL, or Washing), see Parvovirus B19 by PCR, Quantitative [B19PCQ].
Components
| Code | Name |
|---|---|
| B19QLS | B19 Qual Specimen Descrip |
| B19QLR | B19 Qual Result |
| B19QLI | B19 Qual Interp |
Interpretation
Method
Real-time Polymerase Chain Reaction.
Reference Range
See individual componentsGuidelines
Ordering & Collection
Specimen Type
Collection
Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).
Paraffin Tissue: DO NOT SEND SLIDES. Slice 3-4 scrolls that are 5-50 micron thick in sterile tube.
Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube, No Heparin aspirate in syringe.
Swab: In sterile tube/universal transport media (UTM).
Cellular fluid: 1 mL in sterile tube
Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.
Minimum sample requirement: 0.5 mL
Handling Instructions
Bone Marrow: 5 mL bone marrow in EDTA (LAVENDER TOP) tube to Laboratory at room temperature within 24 hour of collection.
Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.
Quantity
requested: See Collection
minimum: 0.5 mL
Processing
Tissue Biopsy: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Paraffin Tissue: Ship at room temperature.
Bone Marrow: Ship at room temperature within 24 hours of collection.
Swabs: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Outside Laboratory:
Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder.
Tissue/Cellular fluid/Swab: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
| CL |
Division of Infectious Disease Diagnostics (DIDD) - Virology
1601 Lind Ave SW |
Phone: 206-685-6656 option 8 |
|---|
Frequency
Wednesday. Results following Tuesday.
Available STAT?
No