B Cell Clonality by PCR
General Information
Lab Name
B Cell Clonality by PCR
Lab Code
BCELL
Epic Name
B Cell Clonality by PCR
Description
Monoclonal populations of B lymphocytes can be identified by testing for immunoglobulin heavy chain (IGH) and kappa light chain gene (IGK) rearrangement by the polymerase chain reaction. Our method, developed by the collaborative European BIOMED-2 Concerted Action study group, employs three multiplex primer sets, each recognizing one of three conserved “Framework Regions” (FR1, FR2, and FR3) of (IGH) variable gene segments (VH) and two multiplex primer sets targeting the kappa light chain locus (IGK).
For patients whose lymphoma has been shown to have a detectable clonal B cell population, this assay can be used in the post-treatment context of B cell depleted specimens. However, this test is not sufficiently sensitive for post-treatment monitoring of B lymphoblastic leukemia; consider send-out testing for next-generation sequencing of IGH.
Sensitivity: This assay detects approximately 90% of B cell clones, provided that the clonal population represents at least 2-5% of the B cells in a specimen.
Hematopathology test request form can be found at Request Form.
Synonyms
B cell gene rearrangement, B-cell clonality analysis, B-cell gene rearrangement, IgH gene clonality, IgH gene rearrangement, IgK gene clonality, IgK gene rearrangement
Components
| Code | Name |
|---|---|
| BCELSD | B Cell Clonality PCR Specimen |
| BCELRS | B Cell Clonality PCR Result |
| BCELIN | B Cell Clonality PCR Interp |
| BCREV | B Cell Clonality Results Reviewed by: |
| BCELM | B Cell Clonality PCR Method |
| BCRGRN | B Cell Clonality Case Number |
Interpretation
Method
Polymerase Chain Reaction (PCR)/Capillary Electrophoresis
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Preferred:
Blood: 6mL in Lavender Top (EDTA)
Bone Marrow: 1-2mL in Lavender Top (EDTA)
Tissue, Fluids or Sorted cells: Sterile container in RPMI
Formalin-fixed paraffin embedded tissue (FFPE/PET) block
Slides: 10 unstained
Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.
Extracted DNA from CLIA certified laboratory.
Formalin-fixed paraffin embedded tissue (FFPE/PET)
Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides
Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Decalcified or unacceptable fixatives
Forms & Requisitions
Handling Instructions
- Deliver samples to Hematopathology as soon as possible.
- If sample is older than 48 hours, please store at 2-6°C upon receipt and send refrigerated.
- Samples stored at room temperature or refrigerated older than 7 days will be rejected.
- Frozen samples should be shipped on dry ice.
Quantity
requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Tissue Blocks: Adequate size to cut 10-20 curls/Slides: 10 unstained
minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/Tissue Blocks: 5 curls/Slides: 5 slides
Processing
Send specimens to Hematopathology – Molecular department for processing.
The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.
Blood and BMA must arrive in lab by 7am and tissue by 12pm Friday to be included on Tuesday run.
Performance
LIS Dept Code
Molecular Hematopathology (MHP)
Performing Location(s)
| Other |
Hematopathology, Molecular
206-606-7060 UW Hematopathology Laboratory, G7-800 |
Hematopathology, Molecular hours: |
|---|
Frequency
Tuesday and Thursday: Results available up to 5 days.
Available STAT?
No