Influenza A, B & RSV PCR Panel
General Information
Lab Name
Influenza A/B & RSV by PCR
Lab Code
FLURSV
Epic Name
Influenza A/B and RSV by PCR
Description
The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® Flu A/B/RSV assay for the rapid detection and differentiation of human influenza A/B, and respiratory syncytial virus (RSV) using real-time PCR.
The assay targets the influenza A viruses (matrix gene), the influenza B viruses (matrix gene) and RSV (matrix gene). Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.
Synonyms
Influenza A, Influenza B, Respiratory syncytial virus (RSV)
Components
| Code | Name |
|---|---|
| FLUSD | Influenza A/B & RSV Specimen |
| FLAPCR | Influenza A PCR Result |
| CTFLA | Influenza A Ct Value |
| FLBPCR | Influenza B PCR Result |
| CTFLB | Influenza B Ct Value |
| RSVPCR | RSV PCR Result |
| CTRSV | RSV Ct Value |
| FLURI | Influenza A/B & RSV Interp |
Interpretation
Method
Real-time Polymerase Chain Reaction.
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Collect swab specimens according to standard technique using a polyester-, rayon-, or nylon-tipped swab. Immediately place the swab specimen into VTM/UTM. Do not use calcium alginate swabs. Refrigerate (2-8°C) within 4 hours of collection.
Collect Broncho alveolar lavage (BAL) and tracheal aspirates in a sterile container.
Minimum sample requirement: 0.75 mL
Handling Instructions
Transport specimens on ice (ice Pak), store at 2 to 8°C for up to 72 hours post collection. If there is a greater than 72 hours delay before testing of the specimen, freeze at -70°C or colder and ship on dry ice. Do not freeze/thaw.
Quantity
requested: Swab in UTM
minimum: 750 µl
Processing
Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
| CL |
Virology, Renton
206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356 |
206-685-6656 opt 4 |
|---|
Frequency
Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.
Available STAT?
No