Parainfluenza PCR Panel
General Information
Lab Name
Parainfluenza PCR Panel
Lab Code
FUSPIV
Epic Name
Parainfluenza PCR Panel
Description
The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® assay for the detection and differentiation of Human parainfluenza viruses type 1, type 2 , type 3 and type 4 using real-time PCR.
The assays targets the hemagglutinin neuraminidase gene for each virus. Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.
Components
| Code | Name |
|---|---|
| FUPSD | Parainfluenza PCR Specimen |
| FUSPV1 | Parainfluenza 1 Result |
| FPV1CT | PIV1 Ct Value |
| FUSPV2 | Parainfluenza 2 Result |
| FPV2CT | PIV2 Ct Value |
| FUSPV3 | Parainfluenza 3 Result |
| FPV3CT | PIV3 Ct Value |
| FUSPV4 | Parainfluenza 4 Result |
| FPV4CT | PIV4 Ct Value |
| FPIVIN | Parainfluenza PCR Interp |
Interpretation
Method
Real-time Polymerase Chain Reaction.
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Collect swab specimens according to standard technique using a polyester-, rayon-, or nylon-tipped swab. Immediately place the swab specimen into VTM/UTM. Do not use calcium alginate swabs. Refrigerate (2-8°C) within 4 hours of collection.
Collect Broncho alveolar lavage (BAL) and tracheal aspirates in a sterile container.
Minimum sample requirement: 0.75 mL
Handling Instructions
Transport specimens on ice (ice Pak), store at 2 to 8°C for up to 72 hours post collection. If there is a greater than 72 hours delay before testing of the specimen, freeze at -70°C or colder and ship on dry ice. Do not freeze/thaw.
Quantity
requested: 1 mL
minimum: 750 µl
Processing
Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
| CL |
Virology, Renton
206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356 |
206-685-6656 opt 4 |
|---|
Frequency
Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.
Available STAT?
No