Human Herpes 6 Virus Chromosome Integration and Typing

General Information

Lab Name

HHV6 Integration and Typing

Lab Code

HH6ABC

Epic Name

HHV6 Chromosome Integration and Typing

Description

This assay detects Human Herpesvirus 6 (HHV-6), determines its subtype, and assesses chromosomal integration using Droplet Digital PCR (ddPCR). ddPCR partitions a bulk reaction into approximately 20,000 individual droplets, each scored as positive or negative for the DNA template (HHV-6), enabling absolute quantification. RPP30, a two-copy gene per diploid human cell, is used to estimate the number of cells in the sample. Chromosomal integration is indicated when the ratio of HHV-6 copies per cell to half of the RPP30 copies per cell (HHV-6 / [RPP30/2]) is approximately 1.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For HHV-6 Quantitation Testing, see HHV6 Quantitative by PCR [HH6QN].

Synonyms

HHV-6, HHV6, HHV6CI

Components

Code	Name
CIHH6A	HHV6 Type A
CIHH6B	HHV6 Type B
HH6VCR	HHV6 Virus to Cell Ratio
HH6CII	HHV6 Chromo Integrat Interp

Interpretation

Method

Droplet Digital PCR

Reference Range

See individual components

Ordering & Collection

Specimen Type

Whole blood

Collection

Blood: 5 mL blood in EDTA (LAVENDER TOP) tube. Send whole blood in room temperature within 24 hours of collection. DO NOT centrifuge or freeze whole blood.

Also acceptable: 5 mL blood in PPT (PEARL TOP) tube

Unacceptable samples: GREEN TOP, RED TOP or GOLD SST tube. DO NOT centrifuge or freeze whole blood.

DNA Specimens: Extracted DNA specimens are acceptable given that their DNA concentration is between 50–100 ng/μL with a volume of at least 100 μL. Extracted DNA must be stored at -20/-80°C and transported to the lab frozen.

The lab only accepts isolated or extracted nucleic acid from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by CAP.

Minimum sample requirement: 1 mL whole blood

Handling Instructions

Send whole blood specimens to the Lab immediately at room temperature.

The Laboratory MUST receive the sample within 48 hours of collection. If sample cannot get to the testing laboratory at room temperature within 24 hours, send whole blood at 2-8°C (ice pack). Sample may be rejected if the testing laboratory is not able to process within 72 hours of collection.

Extracted DNA must be stored at -20°C to -80°C and transported to the lab frozen.

Quantity

requested: 5 mL whole blood
minimum: 1 mL whole blood

Processing

Blood: Hold whole blood at room temperature and send at room temperature. If sample cannot get to the testing laboratory at room temperature within 24 hours, send whole blood at 2-8°C (ice pack). DO NOT centrifuge or freeze whole blood. Samples may be rejected if the testing laboratory is not able to process with in 72 hours of collection.

DNA Specimens: Extracted DNA specimens are acceptable given that their DNA concentration is between 50–100 ng/μl, with a volume of at least 100 μl. Extracted DNA must be stored at -20/-80°C and transported to the lab frozen.

Outside Laboratories: Send whole blood to laboratory at room temperature. If sample cannot get to the testing laboratory at room temperature within 24 hours, send whole blood at 2-8°C (ice pack). Samples received in the testing laboratory greater than 72 hours old may be rejected.

Performance

LIS Dept Code

Central Lab Virology (RVIR)

Performing Location(s)

CL	Infectious Disease Division (IDD) - Virology 1601 Lind Ave SW Renton, WA 98057	Phone: 206-685-6656 option 8 Hours of Operation: Mon–Fri: 7:00 AM–7:00 PM Sat: 9:00 AM–4:00 PM

Laboratory Test Guide