Heme Single Gene by NGS
General Information
Lab Name
Heme Single Gene by NGS
Lab Code
HOTSGH
Epic Name
Heme Single Gene Sequencing
Description
The Heme Single Gene by NGS (HOTSGH) assay is intended to test a single gene selected from the Heme Amplicon panel. See synonyms field above for the list of offered genes. The regions of the genes included are amplified using amplification technology and sequenced on an Illumina instrument to detect single nucleotide variants, insertions, and deletions. This test is designed to detect somatic mutations in the setting of neoplasia, and is not designed to detect germline (heritable) mutations.
Synonyms
11-17, 14, 15, 16), 17), 26, 27), 3, 6), 6), ASXL1 (CDS), CBL (CDS), CSF3R (Exons 14, DNMT3A (CDS), EZH2 (Exons 15-20), FBXW7 (CDS), FGFR1 (Exons 4, FLT3 (p.D835H), GATA1 (Exons 2-3), GATA2 (Exons 3-5), Hotspot, HRAS (Exon 1-2), IDH1( p.R132), IDH2 (Exon 4), JAK2 (Exon 12, KIT (8-18), KMT2A (CDS), KRAS (CDS), MAP2K1 (Exons 2, MPL (Exon 10), MYD88 (Exon 3-5), next-generation sequencing, NGS, NOTCH1 (Exons 20, NPM1 (Exon 12), NRAS (CDS), partial 18), PDGFRA (Exons 12-18), PHF6 (CDS), PTEN (CDS), RB1 (CDS), RUNX1 (Exon 4-8), SF3B1 (Exon 14-16), single gene, SRSF2 (Exon 1), STAG2 (CDS), STAT3 (Exons 20-21), TET2 (CDS), TP53 (CDS), U2AF1 (Exons 2, WT1 (CDS), ZRSR2 (CDS)
Components
| Code | Name |
|---|---|
| SGHSD | Heme Gene Specimen Description |
| SGHRES | Heme Gene Result |
| SGHINT | Heme Gene Interpretation |
| SGHM | Heme Gene Method |
Interpretation
Method
Amplification of the stated genetic regions followed by sequencing using an Illumina instrument
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Blood: 6 mL blood in LAVENDER top (EDTA) tubeBone Marrow: 1-2 mL in a LAVENDER top (EDTA) tube
Handling Instructions
Samples should be refrigerated and delivered to the Laboratory without delay.
Extracted DNA will be accepted only from CLIA certified laboratories.
Quantity
requested: Entire tube
Processing
Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood
Samples submitted outside of these ranges will be evaluated for quality using appropriate internal controls and the results qualified appropriately.
Performance
LIS Dept Code
Molecular Hemepath- OLD (MHPO)
Performing Location(s)
| Other |
Hematopathology, Molecular
206-606-7060 UW Hematopathology Laboratory, G7-800 |
Hematopathology, Molecular hours: |
|---|
Frequency
setup weekly, results in 10-12 days.
Available STAT?
No
Billing & Coding
CPT codes
Billing Comments
For additional test/billing information, see Heme Single Gene by NGS CPT codes.
LOINC
Interfaced Order Code
UOW3081