HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid
General Information
Lab Name
HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid
Lab Code
HS12QN
Epic Name
HSV Type 1 & 2 Quantitative PCR, Blood
Description
The detection and quantitation of Herpes Simplex virus (HSV) by real-time PCR amplification. PCR detects amplicons of the gB gene for both HSV-1 and HSV-2. The two probes used in the reaction, specific either to HSV-1 or HSV-2 are labeled with different reporter dyes. Quantitation of HSV DNA (copies/mL) is achieved by amplifying a standard curve consisting of serial dilutions of a known amount of DNA containing the HSV-1 and HSV-2 amplicons. Results are reported in copies per milliliter (copies/mL). The minimum virus level that gives a positive result in 95% of replicates is 250 copies/mL (2.40 log copies/mL).
This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.
For tissue biopsy, bone marrow, or cellular fluid samples (e.g., BAL or washing containing visible cellular material), qualitative testing should be ordered. In these instances, refer to HSV Type 1 & 2 Qual PCR, Tissue [HS12QL].
For CSF or Swab, see [[RPDHSV]].
Synonyms
Herpes Simplex Virus by PCR, PCR for Herpes Simplex
Components
| Code | Name |
|---|---|
| HSVQNS | HSV Specimen_PCR Quant |
| HSVQN1 | HSV Type 1 Quant PCR Result |
| HSVLO1 | HSV1 DNA Copies/mL (Log 10) |
| HSVQN2 | HSV Type 2 Quant PCR Result |
| HSVLO2 | HSV2 DNA Copies/mL (Log 10) |
| HSVQNI | HSV Interpretation_PCR Quant |
| HSVQNM | HSV Type 1 and 2 Quant PCR Method |
Interpretation
Method
Real-time Polymerase Chain Reaction.
Reference Range
See individual components
Ordering & Collection
Specimen Type
Collection
Blood: 3 mL blood in EDTA (LAVENDER TOP) tube.
Eye Fluid: 50 microliters in sterile container.
NOTE: Do not send syringe with needle. Only send syringe or transfer fluid to sterile container.
Clear non-cellular fluid (e.g. BAL or Washing): 1 mL sterile container.
Also Acceptable: 3 mL blood in GOLD SST, RED, or PPT (PEARL TOP) tube.
Unacceptable samples: Plasma collected in sodium or lithium heparin.
Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.
Minimum sample requirement: 0.5 mL
Quantity
requested: 1.0 mL serum/plasma, 50 microliters eye fluid
minimum: 0.5 mL serum/plasma/non cellular fluid.
Processing
Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Eye Fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Clear non-cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
| Other |
Virology, Molecular
206-685-6656 Virology Laboratory, Ste 320 |
|---|
Frequency
Tuesday and Friday.
Available STAT?
No